Distribution of lymphocytes, immunoglobulin-containing cells, macrophages, and dendritic cells in the accessory sex glands of rams experimentally infected with Actinobacillus seminis
Por:
Acosta-Dibarrat, Jorge, Tenorio-Gutierrez, Victor, Soriano-Vargas, Edgardo, Talavera-Rojas, Martin, Cal-Pereyra, Luis, de Oca Jimenez, Roberto Montes, Velazquez-Ordonez, Valente, Tortora-Perez, Jorge
Publicada:
1 may 2016
Categoría:
Veterinary (miscellaneous)
Resumen:
The distribution of cells involved in the immune response in accessory
sex glands of rams experimentally infected with Actinobacillus seminis
was studied. Twelve one-year old rams were experimentally infected by
intraurethral (IU) (n=4) and intraepididymal (IE) (n=4) route, and four
control (CON) animals were used. The animals were slaughtered 35 days
post-inoculation, samples were taken from accessory sex glands, and
bacteriology and histopathology tests were performed. The presence of
CD4, CD8 and TCR gamma delta (WC1) lymphocytes, CD45RO cells,
macrophages (CD14), dendritic cells (CD1b), IgA-, IgG- and
IgM-containing cells (IgCC) was determined. Animals of the IE group
developed clinical epididymitis. No lesions were seen in rams of the IU
group; two of the intraepididymal inoculated CON developed small lesions
in the epididymis. A. seminis isolates were achieved from 6: 16 (37.5%)
accessory sex glands in the IE group, but not in the IU and CON groups.
In the CON group, IgA- and IgM-containing cells predominated in the
bulbourethral glands and the disseminated prostate, and they were scarce
or null in the vesicles and ampullae. A significant increase of IgA-,
IgG-and IgM-containing cells was confirmed in the seminal vesicles, the
ampullae and the bulbourethral glands in the IE group. In the IE and IU
groups, an increase in CD4, CD8, WC1, CD45RO and CD14 was evidenced in
the vesicles and ampullae. CD1b dendritic cells were present in the
ampullae and vesicles with inflammatory processes. A. seminis triggered
a local immune response in the IE and IU groups. These results indicate
a different pattern of infiltrating immune cells in the accessory sex
glands of infected A. seminis rams.
Filiaciones:
Acosta-Dibarrat, Jorge:
Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Ctr Invest & Estudios Avanzados Salud Anim, Km 15,5 Carretera Toluca Atlacomulco, Toluca 50100, ME, Mexico
Tenorio-Gutierrez, Victor:
Ctr Nacl Invest Disciplinaria Microbiol CENID Mic, INIFAP, Km 15,5 Carretera Fed Mexico, Toluca 05110, DF, Mexico
Soriano-Vargas, Edgardo:
Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Ctr Invest & Estudios Avanzados Salud Anim, Km 15,5 Carretera Toluca Atlacomulco, Toluca 50100, ME, Mexico
Talavera-Rojas, Martin:
Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Ctr Invest & Estudios Avanzados Salud Anim, Km 15,5 Carretera Toluca Atlacomulco, Toluca 50100, ME, Mexico
Cal-Pereyra, Luis:
Univ Republica, Fac Vet, Dept Patol, Alberto Lasplaces 1550, Montevideo 11600, Uruguay
de Oca Jimenez, Roberto Montes:
Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Ctr Invest & Estudios Avanzados Salud Anim, Km 15,5 Carretera Toluca Atlacomulco, Toluca 50100, ME, Mexico
Velazquez-Ordonez, Valente:
Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Ctr Invest & Estudios Avanzados Salud Anim, Km 15,5 Carretera Toluca Atlacomulco, Toluca 50100, ME, Mexico
Tortora-Perez, Jorge:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2,5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54714, ME, Mexico
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