Microbiota from Rhabditis regina may alter nematode entomopathogenicity
Por:
Guillermo Jimenez-Cortes, Jesus, Canales-Lazcano, Jorge, Lara-Reyes, Nancy, Rosenblueth, Monica, Martinez-Romero, Esperanza, Contreras-Garduno, Jorge
Publicada:
1 nov 2016
Resumen:
Here we report the presence of the entomopathogenic nematode Rhabditis
(Rhabditoides) regina affecting white grubs (Phyllophaga sp. and Anomala
sp.) in Mexico and R. regina-associated bacteria. Bioassays were
performed to test the entomopathogenic capacity of dauer and L2 and L3
(combined) larval stages. Furthermore, we determined the diversity of
bacteria from laboratory nematodes cultivated for 2 years (dauer and
L2-L3 larvae) and from field nematodes (dauer and L2-L3 larvae) in
addition to the virulence in Galleria mellonella larvae of some
bacterial species from both laboratory and field nematodes. Dauer and
non-dauer larvae of R. regina killed G. mellonella. Bacteria such as
Serratia sp. (isolated from field nematodes) and Klebsiella sp.
(isolated from larvae of laboratory and field nematodes) may explain R.
regina entomopathogenic capabilities. Different bacteria were found in
nematodes after subculturing in the laboratory suggesting that R. regina
may acquire bacteria in different environments. However, there were some
consistently found bacteria from laboratory and field nematodes such as
Pseudochrobactrum sp., Comamonas sp., Alcaligenes sp., Klebsiella sp.,
Acinetobacter sp., and Leucobacter sp. that may constitute the nematode
microbiome. Results showed that some bacteria contributing to
entomopathogenicity may be lost in the laboratory representing a
disadvantage when nematodes are cultivated to be used for biological
control.
Filiaciones:
Univ Guanajuato, Div Ciencias Nat & Exactas, Dept Biol, Guanajuato, Mexico
Univ Nacl Autonoma Mexico, Lab Biol Parasitos, Fac Med, Mexico City, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City, DF, Mexico
UNAM, Unidad Morelia, Escuela Nacl Estudios Super, Morelia, Michoacan, Mexico
UNAM, Ctr Ciencias Genom, Cuernavaca, Morelos, Mexico
UNAM, LANASE, Escuela Nacl Estudios Super, Unidad Morelia, Morelia, Michoacan, Mexico
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