Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study
Por:
Perez-Ponce De Leon, Gerardo, Garcia-Varela, Martin, Pinacho-Pinacho, Carlos D., Sereno-Uribe, Ana L., Poulin, Robert
Publicada:
1 nov 2016
Resumen:
The recent development of genetic methods allows the delineation of
species boundaries, especially in organisms where morphological
characters are not reliable to differentiate species. However, few
empirical studies have used these tools to delineate species among
parasitic metazoans. Here we investigate the species boundaries of
Clinostomum, a cosmopolitan trematode genus with complex life cycle. We
sequenced a mitochondrial [cytochrome c oxidase subunit I (COI)] gene
for multiple individuals (adults and metacercariae) from Middle-America.
Bayesian phylogenetic analysis of the COI uncovered five reciprocally
monophyletic clades. COI sequences were then explored using the
Automatic Barcode Gap Discovery to identify putative species; this
species delimitation method recognized six species. A subsample was
sequenced for a nuclear gene (ITS1, 5 center dot 8S, ITS2), and a
concatenated phylogenetic analysis was performed through Bayesian
inference. The species delimitation of Middle-American Clinostomum was
finally validated using a multispecies coalescent analysis (species
tree). In total, five putative species are recognized among our samples.
Mapping the second intermediate hosts (fish) onto the species tree
suggests that metacercariae of these five species exhibit some level of
host specificity towards their fish intermediate host (at the family
level), irrespective of geographical distribution.
Filiaciones:
Perez-Ponce De Leon, Gerardo:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ciudad Univ,Ap Postal 70-153, Mexico City 04510, DF, Mexico
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ciudad Univ,Ap Postal 70-153, Mexico City 04510, DF, Mexico
Pinacho-Pinacho, Carlos D.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ciudad Univ,Ap Postal 70-153, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Posgrad Ciencins Biol, Mexico City, DF, Mexico
Sereno-Uribe, Ana L.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ciudad Univ,Ap Postal 70-153, Mexico City 04510, DF, Mexico
Poulin, Robert:
Univ Otago, Dept Zool, POB 56, Dunedin, New Zealand
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