Pseudomonas aeruginosa ATCC 9027 is a non-virulent strain suitable for mono-rhamnolipids production
Por:
Grosso-Becerra, Maria-Victoria, Gonzalez-Valdez, Abigail, Granados-Martinez, Maria-Jessica, Morales, Estefania, Servin-Gonzalez, Luis, Mendez, Jose-Luis, Delgado, Gabriela, Morales-Espinosa, Rosario, Ponce-Soto, Gabriel-Yaxal, Cocotl-Yanez, Miguel, Soberon-Chavez, Gloria
Publicada:
1 dic 2016
Resumen:
Rhamnolipids produced by Pseudomonas aeruginosa are biosurfactants with
a high biotechnological potential, but their extensive commercialization
is limited by the potential virulence of P. aeruginosa and by
restrictions in producing these surfactants in heterologous hosts. In
this work, we report the characterization of P. aeruginosa strain ATCC
9027 in terms of its genome-sequence, virulence, antibiotic resistance,
and its ability to produce mono-rhamnolipids when carrying plasmids with
different cloned genes from the type strain PAO1. The genes that were
expressed from the plasmids are those coding for enzymes involved in the
synthesis of this biosurfactant (rhlA and rhlB), as well as the gene
that codes for the RhlR transcriptional regulator. We confirm that
strain ATCC 9027 forms part of the PA7 clade, but contrary to strain
PA7, it is sensitive to antibiotics and is completely avirulent in a
mouse model. We also report that strain ATCC 9027 mono-rhamnolipid
synthesis is limited by the expression of the rhlAB-R operon. Thus, this
strain carrying the rhlAB-R operon produces similar rhamnolipids levels
as PAO1 strain. We determined that strain ATCC 9027 with rhlAB-R operon
was not virulent to mice. These results show that strain ATCC 9027,
expressing PAO1 rhlAB-R operon, has a high biotechnological potential
for industrial mono-rhamnolipid production.
Filiaciones:
Grosso-Becerra, Maria-Victoria:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Ciudad Univ,Apartado Postal 70228, Mexico City 04510, DF, Mexico
Gonzalez-Valdez, Abigail:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Ciudad Univ,Apartado Postal 70228, Mexico City 04510, DF, Mexico
Granados-Martinez, Maria-Jessica:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Ciudad Univ,Apartado Postal 70228, Mexico City 04510, DF, Mexico
Morales, Estefania:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Ciudad Univ,Apartado Postal 70228, Mexico City 04510, DF, Mexico
Servin-Gonzalez, Luis:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Ciudad Univ,Apartado Postal 70228, Mexico City 04510, DF, Mexico
Mendez, Jose-Luis:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ciudad Univ, Mexico City 04510, DF, Mexico
Delgado, Gabriela:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ciudad Univ, Mexico City 04510, DF, Mexico
Morales-Espinosa, Rosario:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ciudad Univ, Mexico City 04510, DF, Mexico
Ponce-Soto, Gabriel-Yaxal:
Univ Nacl Autonoma Mexico, Inst Ecol, Dept Ecol Evolut, Ciudad Univ, Mexico City 04510, DF, Mexico
Cocotl-Yanez, Miguel:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Ciudad Univ,Apartado Postal 70228, Mexico City 04510, DF, Mexico
Soberon-Chavez, Gloria:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Ciudad Univ,Apartado Postal 70228, Mexico City 04510, DF, Mexico
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