The cell wall DUF642 At2g41800 (TEB) protein is involved in hypocotyl cell elongation
Por:
Salazar-Iribe, Alexis, Teresa Agredano-Moreno, Lourdes, Zuniga-Sanchez, Esther, Felipe Jimenez-Garcia, Luis, Gamboa-deBuen, Alicia
Publicada:
1 dic 2016
Resumen:
In plants, the cell wall is a complex and dynamic structure comprising
high molecular weight carbohydrates and proteins. The cell wall plays an
important role in several stages of the plant life cycle, including cell
division, elongation and differentiation. The DUF642 family of cell wall
proteins is highly conserved in spermatophytes and might be involved in
pectin structural modifications. Particularly, At2g41800 is one of the
most highly induced genes during the M/G1 phases of the cell cycle, and
the protein encodes by this gene has been detected in cell wall
proteomes of cell suspension cultures. In the present study, the
expression of At2g41800 (TEB) was confirmed in primary and lateral
roots, stigmatic papillae and hypocotyls. Subcellular localization
studies showed that TEB is located in the cell wall. The root length and
lateral root density were not affected in either of the two teb mutants
studied, but the length of the hypocotyls from seedlings grown under
light and dark conditions was increased. Immunogold labelling studies
using JIM5 antibodies on sections of hypocotyl epidermal cells showed an
important reduction of gold particles in teb mutants. The results
suggested that TEB is involved in hypocotyl elongation. (C) 2016
Elsevier Ireland Ltd. All rights reserved.
Filiaciones:
Salazar-Iribe, Alexis:
Univ Nacl Autonoma Mexico, Inst Ecol, Ciudad Univ, Mexico City 04510, DF, Mexico
Teresa Agredano-Moreno, Lourdes:
Univ Nacl Autonoma Mexico, Fac Ciencias, Ciudad Univ, Mexico City 04510, DF, Mexico
Zuniga-Sanchez, Esther:
Univ Nacl Autonoma Mexico, Inst Ecol, Ciudad Univ, Mexico City 04510, DF, Mexico
Felipe Jimenez-Garcia, Luis:
Univ Nacl Autonoma Mexico, Fac Ciencias, Ciudad Univ, Mexico City 04510, DF, Mexico
Gamboa-deBuen, Alicia:
Univ Nacl Autonoma Mexico, Inst Ecol, Ciudad Univ, Mexico City 04510, DF, Mexico
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