The HPV16 E7 oncoprotein increases the expression of Oct3/4 and sternness-related genes and augments cell self-renewal
Por:
Organista-Nava, Jorge, Gomez-Gomez, Yazmin, Ocadiz-Delgado, Rodolfo, Garcia-Villa, Enrique, Bonilla-Delgado, Jose, Lagunas-Martinez, Alfredo, Santa-Olalla Tapia, Jesus, Lambert, Paul F., Garcia-Carranca, Alejandro, Gariglio, Patricio
Publicada:
1 dic 2016
Categoría:
Virology
Resumen:
Oct3/4 is a transcription factor involved in maintenance of the
pluripotency and self-renewal of stem cells. The E7 oncoprotein and 17
beta-estradiol (E-2) are key factors in cervical carcinogenesis. In the
present study, we aimed to investigate the effect of the HPV16 E7
oncoprotein and E-2 on the expression pattern of Oct3/4, Sox2, Nanog and
Fgf4. We also determined whether the E7 oncoprotein is associated with
cell self-renewal. The results showed that Oct3/4, Sox2, Nanog and Fgf4
were upregulated by the E7 oncoprotein in vivo and in vitro and
implicate E-2 in the upregulation of these factors in vivo. We also
demonstrated that E7 is involved in cell self-renewal, suggesting that
the HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4
expression to maintain the self-renewal capacity of cancer stem cells.
(C) 2016 Elsevier Inc. All rights reserved.
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Programa Doctorado Ciencias Biomed, Mexico City 04510, DF, Mexico
Inst Politecn Nacl CINVESTAV IPN, Ctr Invest & Estudios Avanzados, Dept Genet & Biol Mol, Mexico City 07360, DF, Mexico
Hosp Juarez Mexico, Unidad Invest, Mexico City 07760, DF, Mexico
Inst Nacl Salud Publ, CISEI, Div Biol Mol Patogenos, Cuernavaca, Morelos, Mexico
Hosp Nino & Adolescente Morelense, Unidad Diagnost & Med Mol, Cuernavaca, Morelos, Mexico
Univ Autonoma Estado Morelos, Fac Med, Cuernavaca, Morelos, Mexico
Univ Wisconsin, McArdle Lab Canc Res, Sch Med & Publ Hlth, 1400 Univ Ave, Madison, WI 53706 USA
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Unidad Invest Biomed Canc, Mexico City 14080, DF, Mexico
Inst Nacl Cancerol INCan, Secretaria Salud, Div Invest Basica, Mexico City 14080, DF, Mexico
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