Tissue and cellular characterisation of nucleolin in a murine model of corneal angiogenesis
Por:
Quiroz-Mercado, Joaquin, Ramirez-Velazquez, Norma, Partido, Graciela, Zenteno, Edgar, Chavez, Raol, Agundis-Mata, Concepcion, Carmen Jimenez-Martinez, Maria, Garfias, Yonathan
Publicada:
1 sep 2016
Resumen:
Corneal neovascularisation (CNV), with consequent loss of transparency,
is due to an imbalance of proangiogenic factors. Cell-surface nucleolin
(NCL) has been associated with neo-angiogenesis. There are studies
identifying NCL translocation from nucleus to the cell surface, which is
essential for endothelial cell proliferation. To find the possible role
of NCL in the generation of corneal neovessels, the aim of this study is
to characterise the NCL presence and cell-localisation in non-injured
corneas, as well as to describe the changes in NCL cell and tissue
localisation in CNV, and to analyse the effect of bevacizumab on NCL
cellular and tissular distribution.
Suture-induced CNV was performed in mice. The corneal tissues were
obtained and the histological and co-immunofluorescence assays were
performed using different proteins, such as CD31, cadherin and isolectin
B-4. To determine the possible role of VEGF in NCL presence and
localisation in our CNV model, bevacizumab was concomitantly used.
Nucleolin was principally observed in the nucleus of the basal
epithelial cells of normal corneas. Interestingly, angiogenesis-induced
changes were observed in the localisation of NCL, not only in tissue but
also at the cellular level where NCL was extranuclear in epithelial
cells, stromal cells and neovessels. In contrast, these changes were
reverted when bevacizumab was used. Besides, NCL was able to stain only
aberrant corneal neovessels in comparison with retinal vessels.
NCL mobilisation outside the nucleus during angiogenesis could have a
possible role as a proangiogenic molecule in the corneal tissue.
Filiaciones:
Quiroz-Mercado, Joaquin:
Conde de Valenciana Fdn, Inst Ophthalmol, Res Unit, Chimalpopoca 14, Mexico City 06800, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Vet Med & Anim Husb, Ave Univ 3000, Mexico City 04510, DF, Mexico
Ramirez-Velazquez, Norma:
Conde de Valenciana Fdn, Inst Ophthalmol, Res Unit, Chimalpopoca 14, Mexico City 06800, DF, Mexico
Partido, Graciela:
Conde de Valenciana Fdn, Inst Ophthalmol, Res Unit, Chimalpopoca 14, Mexico City 06800, DF, Mexico
Zenteno, Edgar:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Ave Univ 3000, Mexico City 04510, DF, Mexico
Chavez, Raol:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Ave Univ 3000, Mexico City 04510, DF, Mexico
Agundis-Mata, Concepcion:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Ave Univ 3000, Mexico City 04510, DF, Mexico
Carmen Jimenez-Martinez, Maria:
Conde de Valenciana Fdn, Inst Ophthalmol, Res Unit, Chimalpopoca 14, Mexico City 06800, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Ave Univ 3000, Mexico City 04510, DF, Mexico
Garfias, Yonathan:
Conde de Valenciana Fdn, Inst Ophthalmol, Res Unit, Chimalpopoca 14, Mexico City 06800, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Ave Univ 3000, Mexico City 04510, DF, Mexico
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