In silico clustering of Salmonella global gene expression data reveals novel genes co-regulated with the SPI-1 virulence genes through HilD
Por:
Martinez-Flores, Irma, Perez-Morales, Deyanira, Sanchez-Perez, Mishael, Paredes, Claudia C., Collado-Vides, Julio, Salgado, Heladia, Bustamante, Victor H.
Publicada:
25 nov 2016
Categoría:
Multidisciplinary
Resumen:
A wide variety of Salmonella enterica serovars cause intestinal and
systemic infections to humans and animals. Salmonella Patogenicity
Island 1 (SPI-1) is a chromosomal region containing 39 genes that have
crucial virulence roles. The AraC-like transcriptional regulator HilD,
encoded in SPI-1, positively controls the expression of the SPI-1 genes,
as well as of several other virulence genes located outside SPI-1. In
this study, we applied a clustering method to the global gene expression
data of S. enterica serovar Typhimurium from the COLOMBOS database; thus
genes that show an expression pattern similar to that of SPI-1 genes
were selected. This analysis revealed nine novel genes that are
co-expressed with SPI-1, which are located in different chromosomal
regions. Expression analyses and protein-DNA interaction assays showed
regulation by HilD for six of these genes: gtgE, phoH, sinR, SL1263
(lpxR) and SL4247 were regulated directly, whereas SL1896 was regulated
indirectly. Interestingly, phoH is an ancestral gene conserved in most
of bacteria, whereas the other genes show characteristics of genes
acquired by Salmonella. A role in virulence has been previously
demonstrated for gtgE, lpxR and sinR. Our results further expand the
regulon of HilD and thus identify novel possible Salmonella virulence
genes.
Filiaciones:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Computac, Cuernavaca 62210, Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Microbiol Mol, Cuernavaca, Morelos 62210, Mexico
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