Bioactivity of an antihypertensive peptide expressed in Chlamydomonas reinhardtii
Por:
Estefania Ochoa-Mendez, Celma, Lara-Hernandez, Ignacio, Martinez Gonzalez, Luzmila, Aguirre-Banuelos, Patricia, Ibarra-Barajas, Maximiliano, Castro-Moreno, Patricia, Gonzalez-Ortega, Omar, Elena Soria-Guerra, Ruth
Publicada:
20 dic 2016
Resumen:
In this study, we developed a transplastomic C. reinhardtii strain that accumulates anti-hypertensive peptides. Tandem repeats of VLPVP peptide were included. PCR analysis confirmed the presence of the transgene in the modified strains. After in vitro digestion of biomass of a recombinant C. reinhardtii strain the VLVPV peptide was identified and quantified by HPLC. The highest expression line produced 0.292 mg of recombinant protein per mg of freeze-dried biomass. Intragastric administration of the genetically modified strain to spontaneous hypertensive rats at a dose of 30 mg/kg of body weight of recombinant protein significantly reduced systolic blood pressure. At the same dose, the recombinant protein exerts an ACE-inhibitory effect. This is the first study that indicates the potential of this microalga producing an antihypertensive peptide as a dietary supplement for hypertension patients. © 2016 Elsevier B.V.
Filiaciones:
Estefania Ochoa-Mendez, Celma:
Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Av Dr Manuel Nava 6, Slp 78210, Mexico
Lara-Hernandez, Ignacio:
Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Av Dr Manuel Nava 6, Slp 78210, Mexico
Martinez Gonzalez, Luzmila:
Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Av Dr Manuel Nava 6, Slp 78210, Mexico
Aguirre-Banuelos, Patricia:
Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Av Dr Manuel Nava 6, Slp 78210, Mexico
Ibarra-Barajas, Maximiliano:
Univ Nacl Autonoma Mexico, FES Iztacala, UBIMED, Av Barrios 1, Tlalnepantla 54090, Mexico
Castro-Moreno, Patricia:
Univ Nacl Autonoma Mexico, FES Iztacala, UBIMED, Av Barrios 1, Tlalnepantla 54090, Mexico
Gonzalez-Ortega, Omar:
Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Av Dr Manuel Nava 6, Slp 78210, Mexico
Elena Soria-Guerra, Ruth:
Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Av Dr Manuel Nava 6, Slp 78210, Mexico
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