Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays
Por:
Alfonso-Gordillo, Guadalupe, Flores-Ortiz, Cesar Mateo, Morales-Barrera, Liliana, Cristiani-Urbina, Eliseo
Publicada:
1 dic 2016
Resumen:
This study investigated the aerobic biodegradation of methyl
tertiary-butyl ether (MTBE) by a microbial consortium in a continuous
up-flow packed-bed biofilm reactor using tezontle stone particles as a
supporting material for the biofilm. Although MTBE is toxic for
microbial communities, the microbial consortium used here was able to
resist MTBE loading rates up to 128.3 mg L-1 h(-1), with removal
efficiencies of MTBE and chemical oxygen demand (COD) higher than 90%.
A linear relationship was observed between the MTBE loading rate and the
MTBE removal rate, as well as between the COD loading rate and the COD
removal rate, within the interval of MTBE loading rates from 11.98 to
183.71 mg L-1 h(-1). The metabolic intermediate tertiary butyl alcohol
(TBA) was not detected in the effluent during all reactor runs, and the
intermediate 2-hydroxy butyric acid (2-HIBA) was only detected at MTBE
loading rates higher than 128.3 mg L-1 h(-1). The results of toxicity
bioassays with organisms from two different trophic levels revealed that
the toxicity of the influent was significantly reduced after treatment
in the packed-bed reactor. The packed-bed reactor system used in this
study was highly effective for the continuous biodegradation of MTBE and
is therefore a promising alternative for detoxifying MTBE-laden
wastewater and groundwater.
Filiaciones:
Alfonso-Gordillo, Guadalupe:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Ingn Bioquim, Mexico City, DF, Mexico
Flores-Ortiz, Cesar Mateo:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biotecnol & Prototipos, Tlalnepantla, Estado De Mexic, Mexico
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Lab Nacl Salud, Tlalnepantla, Estado De Mexic, Mexico
Morales-Barrera, Liliana:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Ingn Bioquim, Mexico City, DF, Mexico
Cristiani-Urbina, Eliseo:
Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Ingn Bioquim, Mexico City, DF, Mexico
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