Entamoeba histolytica and Entamoeba dispar infection in Mexican school children: genotyping and phylogenetic relationship
Por:
Rojas, Liliana, Moran, Patricia, Valadez, Alicia, Gomez, Alejandro, Gonzalez, Enrique, Hernandez, Eric, Partida, Oswaldo, Nieves, Miriam, Gudino, Marco, Magana, Ulises, Torres, Javier, Ximenez, Cecilia
Publicada:
13 sep 2016
Categoría:
Infectious Diseases
Resumen:
Background: This study aimed to determine the frequency of Entamoeba
histolytica and Entamoeba dispar infection in school children in the
community of Tlaltizapan, in order to understand the dynamics of
infection within the school and family spheres of this population.
Amoebiasis is an unsolved public health problem and an endemic disease
in Mexico. The incidence rate varies depending on the state; the most
affected states show the highest numbers of new cases of amoebiasis per
year. Previously, we reported the molecular frequency of infection with
E. histolytica and/or E. dispar in other rural communities of the state
of Morelos.
Methods: Children from 3 schools were studied to estimate the frequency
of intestinal parasites through microscopic examination of fresh stool
samples. The number of studied individuals were 309 school children. The
molecular characterization of E. histolytica or E. dispar was carried
out by Polymerase Chain Reaction (PCR) using species-specific primers to
amplify short tandem repeats (STR) in non-coding sequences associated
with the tRNA gene; the amplified fragments were sequenced and analyzed.
Results: Eight different genotypes were obtained from E. dispar isolates
with the molecular marker NKD3-D5. None of the cases in which the
species E. histolytica was detected developed symptoms attributable to
an invasive process of disease. Moreover, the parasitized condition
appeared to have no significant impact on the development or nutritional
status of affected children. Genotype 1, which corresponds to the
reference strain E. dispar SAW760, considered a non-pathogenic amoeba,
was the most prevalent.
Conclusions: The comparison of the genotypes of Entamoeba species did
not show a correlation between children and their relatives. In this
community, the species Entamoeba dispar genotype 1 was the most
widespread. Based on the indicators of growth, development and nutrition
status, the studied community seems to be reasonably adapted to constant
exposure to intestinal parasites, since there were no evidences of a
serious impact of the parasitized condition on the children's health.
Filiaciones:
Rojas, Liliana:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Moran, Patricia:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Valadez, Alicia:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Gomez, Alejandro:
IMSS, Res Unit Infect Dis, Pediat Hosp, Century Med Ctr 21, Mexico City, DF, Mexico
Gonzalez, Enrique:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Hernandez, Eric:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Partida, Oswaldo:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Nieves, Miriam:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Gudino, Marco:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Magana, Ulises:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
Torres, Javier:
IMSS, Res Unit Infect Dis, Pediat Hosp, Century Med Ctr 21, Mexico City, DF, Mexico
Ximenez, Cecilia:
Natl Autonomous Univ Mexico UNAM, Res Unit Expt Med, Fac Med, Dr Balmis 148 Col Doctores, Mexico City 06726, DF, Mexico
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