Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants
Por:
Sánchez-Rojo S., Cerda-García-Rojas C.M., Esparza-García F., Plasencia J., Poggi-Varaldo H.M., Ponce-Noyola T., Ramos-Valdivia A.C.
Publicada:
1 dic 2015
Categoría:
Biotechnology
Resumen:
Objective: To obtain micro propagated Uncaria tomentosa plantlets with enhanced secondary metabolites production, long-term responses to salicylic acid (SA) pre-treatments at 1 and 100 µM were evaluated after propagation of the plantlets in a SA-free medium. Results: SA pre-treatments of single node cuttings OF U. tomentosa produced long-term responses in microplants grown for 75 days in a SA-free medium. Reduction in survival rate, root formation, and stem elongation were observed only with 100 µM SA pre-treatments with respect to the control (0 + DMSO).Both pre-treatments enhanced H 2 O 2 and inhibited superoxide dismutase and catalase activities, while guaiacol peroxidase was increased only with 1 µM SA. Also, both pre-treatments increased total monoterpenoid oxindole alkaloids by ca. 55 % (16.5 mg g -1 DW), including isopteropodine, speciophylline, mitraphylline, isomitraphylline, rhynchopylline, and isorhynchopylline; and flavonoids by ca. 21 % (914 µg g -1 DW), whereas phenolic compounds were increased 80 % (599 µg g -1 DW) at 1 µM and 8.2 % (359 µg g -1 DW) at 100 µM SA. Conclusion: Pre-treatment with 1 µM SA of U.tomentosa microplants preserved the survival rate and increased oxindole alkaloids, flavonoids, and phenolic compounds in correlation with H 2 O 2 and peroxidase activity enhancements, offering biotechnological advantages over non-treated microplants. © 2015, Springer Science+Business Media Dordrecht.
Filiaciones:
Sánchez-Rojo S.:
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, Mexico, D.F., 07360, Mexico
Cerda-García-Rojas C.M.:
Departamento de Química, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, Mexico, D.F., 07360, Mexico
Esparza-García F.:
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, Mexico, D.F., 07360, Mexico
Plasencia J.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Bioquim, Mexico City 04510, DF, Mexico
Poggi-Varaldo H.M.:
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, Mexico, D.F., 07360, Mexico
Ponce-Noyola T.:
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, Mexico, D.F., 07360, Mexico
Ramos-Valdivia A.C.:
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, Mexico, D.F., 07360, Mexico
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