Absorbable bioprosthesis for the treatment of bile duct injury in an experimental model
Por:
Montalvo-Javé E.E., Barrera, GEM, Trevino, AIV, Barba, MCP, Montalvo-Arenas C., Mendoza, FR, Mancilla, BL, Pineda, MAG, Limon, JA, Saavedra, JA, Tapia-Jurado J.
Publicada:
1 ago 2015
Categoría:
Surgery
Resumen:
Introduction: Cholecystectomy is a common surgical procedure in which
complications may occur, such as injury to the biliary tract, which are
associated with high morbidity. The aim of this study was to demonstrate
the efficacy of a polymer-based absorbable bioprosthesis with bone
scaffold for the treatment of bile duct injury in an animal model.
Materials and methods: An absorbable bioprosthesis was used to replace
the common bile duct in 15 pigs which were divided into 3 groups with
different follow-ups at 1, 3 and 6 months. The animals were anesthetized
at these time points and laboratory tests, Magnetic Resonance
Cholangiopancreatogram [MRCP], Choledochoscopy using Spyglass and
Endoscopic retrograde Cholangiopancreatogram [ERCP] were performed.
After radiological evaluation was complete, the animals were euthanized
and histological and immunohistochemical analyses were performed.
Results: Liver function tests at different time points demonstrated no
significant changes. No mortality or postoperative complications were
found in any of the experimental models. Imaging studies ([MRCP],
[ERCP] and Choledochoscopy with SpyGlass (TM)) showed absence of
stenosis or obstruction in all the experimental models.
Discussion: Histological and immunohistochemical staining (CK19 and
MUC5+) revealed the presence of biliary epithelium with intramural
biliary glands in all the experimental models. There was no stenosis or
obstruction in the bile duct.
Conclusions: The bioprosthesis served as scaffolding for tissue
regeneration. There was no postoperative complication at 6 months
follow-up. This bioprosthesis could be used to replace the bile duct in
cancer or bile duct injury. The bioprosthesis may allow different
modeling depending on the type of bile duct injury. (C) 2015 IJS
Publishing Group Limited. Published by Elsevier Ltd. All rights
reserved.
Filiaciones:
Montalvo-Javé E.E.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Barrera, GEM:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Trevino, AIV:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Barba, MCP:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Lab Biomat, Mexico City, DF, Mexico
Montalvo-Arenas C.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biol Celular & Tisular, Mexico City, DF, Mexico
Mancilla, BL:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Pineda, MAG:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Limon, JA:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Tapia-Jurado J.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Cirugia, Mexico City, DF, Mexico
Bronze
|