Motility of Fasciola hepatica miracidia assessed with a computer-assisted sperm analyser
Por:
Villa-Mancera A., Reynoso-Palomar A., Olivares-Pérez J., Ortega-Vargas S., Cruz-Mendoza I., Quiroz-Romero H.
Publicada:
1 jul 2015
Resumen:
The motility parameters of Fasciola hepatica miracidia were assessed at different temperatures and times post-hatching using computer-assisted sperm analysis. Eggs were incubated at 22°C or 25°C for 14 days. Five motion parameters were evaluated at different incubation temperatures up to 10 h post-hatching. No differences were observed in the percentage that hatched after incubation at the two different temperatures. However, the straight-line velocity of miracidia following incubation at 22°C was significantly different from that observed at 25°C (P< 0.01). All miracidium motion parameters at different post-hatching temperatures showed an overall decrease at the end of the experiment. Those miracidia hatching from eggs incubated at 25°C had a higher velocity of 1673.3 µm/s compared with 1553.3 µm/s at 22°C. Velocity parameters increased as the post-hatching temperature increased from 22°C to 37°C. © Cambridge University Press 2014.
Filiaciones:
Villa-Mancera A.:
Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, Tecamachalco Puebla, Mexico
Reynoso-Palomar A.:
Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, Tecamachalco Puebla, Mexico
Olivares-Pérez J.:
Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Ciudad Altamirano, Guerrero, Mexico
Ortega-Vargas S.:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Mexico City 04510, DF, Mexico
Cruz-Mendoza I.:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Mexico City 04510, DF, Mexico
Quiroz-Romero H.:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Mexico City 04510, DF, Mexico
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