Evaluation of the estrogenic and oxidative stress effects of the UV filter 3-benzophenone in zebrafish (Danio rerio) eleuthero-embryos
Por:
Rodríguez-Fuentes G., Sandoval-Gío J.J., Arroyo-Silva A., NorenaBarroso, E, Escalante-Herrera K.S., Olvera-Espinosa F.
Publicada:
1 may 2015
Resumen:
Personal care products have been detected in superficial waters, representing an environmental risk to the biota. Some studies indicated that 3-benzophenone (3BP) alters hormones, inducing vitellogenesis and having adverse effects on fish reproduction. Other studies have reported generation of free radicals and changes in antioxidant enzymes. Therefore, the aim of the present study was to test acute exposure to 3BP at concentrations within and beyond that found environmentally to provide important toxicological information regarding this chemical. We evaluated the effect of 3BP on vitellogenin 1 (VTG1) gene expression and the transcription of the enzymes catalase (CAT), superoxide dismutase (SOD) or glutathione peroxidase (GPx), which are involved in cellular redox balance. Zebrafish eluthero-embryos (168. hpf) were exposed to 1,10, 100, 1000. µg/L 3BP, in addition to a negative control and a 0.1% ethanol control for 48. h. The results of our study indicated a positive significant correlation between exposure concentrations and VTG1 expression (. r=0.986, p=0.0028) but only 1000. µg/L 3BP produced a significant increase from control. Acute exposure showed no significant differences in transcription levels of CAT, SOD or GPx at the tested conditions. Nevertheless, a trend toward increase in GPx expression was observed as a positive significant correlation (. r=0.928, p=0.017) was noted. © 2015.
Filiaciones:
Rodríguez-Fuentes G.:
Univ Nacl Autonoma Mexico, Fac Quim, Sisal, Yucatan, Mexico
Sandoval-Gío J.J.:
Univ Nacl Autonoma Mexico, Fac Quim, Sisal, Yucatan, Mexico
Arroyo-Silva A.:
Benemérita Universidad Autónoma de Puebla, Puebla, Puebla, Mexico
NorenaBarroso, E:
Univ Nacl Autonoma Mexico, Fac Quim, Sisal, Yucatan, Mexico
Escalante-Herrera K.S.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Sisal, Yucatan, Mexico
Olvera-Espinosa F.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Sisal, Yucatan, Mexico
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