Superoxide anion production and expression of gp91phox and p47phox are increased in glomeruli and proximal tubules of cisplatin-treated rats
Por:
Trujillo J., Molina-Jijón E., Medina-Campos O.N., Rodríguez-Muñoz R., Reyes J.L., Barrera D., Pedraza-Chaverri J.
Publicada:
1 abr 2015
Resumen:
The chemotherapeutic drug cisplatin has some side effects including nephrotoxicity that has been associated with reactive oxygen species production, particularly superoxide anion. The major source of superoxide anion is nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase. However, the specific segment of the nephron in which superoxide anion is produced has not been identified. Rats were sacrificed 72 h after cisplatin injection (7.5 mg/kg), and kidneys were obtained to isolate glomeruli and proximal and distal tubules. Cisplatin induced superoxide anion production in glomeruli and proximal tubules but not in distal tubules. This enhanced superoxide anion production was prevented by diphenylene iodonium, an inhibitor of NADPH oxidase. Consistently, this effect was associated with the increased expression of gp91phox and p47phox, subunits of NADPH oxidase. The enhanced superoxide anion production in glomeruli and proximal tubules, associated with the increased expression of gp91phox and p47phox, is involved in the oxidative stress in cisplatin-induced nephrotoxicity. © 2014 Wiley Periodicals, Inc.
Filiaciones:
Trujillo J.:
Univ Nacl Autonoma Mexico, Fac Chem, Dept Biol, Mexico City 04510, DF, Mexico
Molina-Jijón E.:
Department of Physiology, Biophysics and Neuroscience, Center for Research and Advanced Studies, National Polytechnic Institute (Cinvestav-IPN), Mexico, D. F., Mexico
Medina-Campos O.N.:
Univ Nacl Autonoma Mexico, Fac Chem, Dept Biol, Mexico City 04510, DF, Mexico
Rodríguez-Muñoz R.:
Department of Physiology, Biophysics and Neuroscience, Center for Research and Advanced Studies, National Polytechnic Institute (Cinvestav-IPN), Mexico, D. F., Mexico
Reyes J.L.:
Department of Physiology, Biophysics and Neuroscience, Center for Research and Advanced Studies, National Polytechnic Institute (Cinvestav-IPN), Mexico, D. F., Mexico
Barrera D.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Pharmacol, Mexico City 04510, DF, Mexico
Pedraza-Chaverri J.:
Univ Nacl Autonoma Mexico, Fac Chem, Dept Biol, Mexico City 04510, DF, Mexico
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