Unremitting Cell Proliferation in the Secretory Phase of Eutopic Endometriosis: Involvement of pAkt and pGSK3 beta
Por:
Franco-Murillo Y., Miranda-Rodríguez J.A., Rendón-Huerta E., Montano, LF, Cornejo G.V., Gómez L.P., Valdez-Morales F.J., Gonzalez-Sanchez I., Cerbón M.
Publicada:
1 abr 2015
Categoría:
Obstetrics and Gynecology
Resumen:
Objective: Endometriosis is linked to altered cell proliferation and
stem cell markers c-kit/stem cell factor (SCF) in ectopic endometrium.
Our aim was to investigate whether c-kit/SCF also plays a role in
eutopic endometrium.
Design: Eutopic endometrium obtained from 35 women with endometriosis
and 25 fertile eumenorrheic women was analyzed for in situ expression of
SCF/c-kit, Ki67, RAC-alpha serine/threonine-protein kinase (Akt),
phosphorylated RAC-alpha serine/threonin-protein kinase (pAkt), Glycogen
synthase kinase 3 beta (GSK3), and phosphorylated glycogen synthase
kinase 3 beta (pGSK3), throughout the menstrual cycle.
Results: Expression of Ki67 and SCF was higher in endometriosis than in
control tissue (P < .05) and greater in secretory rather than
proliferative (P < .01) endometrium in endometriosis. Expression of
c-kit was also higher in endometriosis although similar in both phases.
Expression of Akt and GSK3 was identical in all samples and cycle
phases, whereas pAkt and pGSK3, opposed to control tissue, remained
overexpressed in the secretory phase in endometriosis.
Conclusion: Unceasing cell proliferation in the secretory phase of
eutopic endometriosis is linked to deregulation of c-kit/SCF-associated
signaling pathways.
Filiaciones:
Univ Nacl Autonoma Mexico, Dept Biol, Fac Quim, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Biol Celular & Tisular, Mexico City 04510, DF, Mexico
Unidad de Endoscopia e Infertilidad, Hospital de la Mujer, Secretaria de Salud, Mexico, Mexico
Servicio de Ginecología y Obstetricia, Hospital Español, Mexico, Federal District, 04510, Mexico
All Open Access; Green
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