Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions
Por:
Cime-Castillo J., Delannoy P., Mendoza-Hernández G., Monroy-Martínez V., Harduin-Lepers A., Lanz-Mendoza H., HernandezHernandez, FD, Zenteno E., Cabello-Gutiérrez C., Ruiz-Ordaz B.H.
Publicada:
1 ene 2015
Resumen:
Dengue fever (DF) is the most prevalent arthropod-borne viral disease
which affects humans. DF is caused by the four dengue virus (DENV)
serotypes, which are transmitted to the host by the mosquito Aedes
aegypti that has key roles in DENV infection, replication, and viral
transmission (vector competence). Mosquito saliva also plays an
important role during DENV transmission. In this study, we detected the
presence of sialic acid (Sia) in Aedes aegypti tissues, which may have
an important role during DENV-vector competence. We also identified
genome sequences encoding enzymes involved in Sia pathways. The cDNA for
Aedes aegypti CMP-Sia synthase (CSAS) was amplified, cloned, and
functionally evaluated via the complementation of LEC29.Lec32
CSAS-deficient CHO cells. AedesCSAS-transfected LEC29.Lec32 cells were
able to express Sia moieties on the cell surface. Sequences related to
alpha-2,6-sialyltransferase were detected in the Aedes aegypti genome.
Likewise, we identified Sia-alpha-2,6-DENV interactions in different
mosquito tissues. In addition, we evaluated the possible role of
sialylated molecules in a salivary gland extract during DENV
internalization in mammalian cells. The knowledge of early DENV-host
interactions could facilitate a better understanding of viral tropism
and pathogenesis to allow the development of new strategies for
controlling DENV transmission.
Filiaciones:
Cime-Castillo J.:
Univ Nacl Autonoma Mexico, Biomed Res Inst, Dept Mol Biol & Biotechnol, UNAM, Mexico City 04510, DF, Mexico
Molecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), México City, 04510, Mexico
Delannoy P.:
Structural and Functional Glycobiology Unit, UMR 8576 CNRS, University of Sciences and Technologies of Lille, Villeneuve d'Ascq, 59655, France
Mendoza-Hernández G.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Mexico City 04510, DF, Mexico
Biochemistry Department, Faculty of Medicine, UNAM, México City, 04510, Mexico
Monroy-Martínez V.:
Univ Nacl Autonoma Mexico, Biomed Res Inst, Dept Mol Biol & Biotechnol, UNAM, Mexico City 04510, DF, Mexico
Molecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), México City, 04510, Mexico
Harduin-Lepers A.:
Structural and Functional Glycobiology Unit, UMR 8576 CNRS, University of Sciences and Technologies of Lille, Villeneuve d'Ascq, 59655, France
Lanz-Mendoza H.:
CISEI, National Institute of Public Health, Cuernavaca, MOR, 62100, Mexico
Zenteno E.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biochem, Mexico City 04510, DF, Mexico
Biochemistry Department, Faculty of Medicine, UNAM, México City, 04510, Mexico
Cabello-Gutiérrez C.:
Virology Department, National Respiratory Institute (INER), México City, 14050, Mexico
Ruiz-Ordaz B.H.:
Univ Nacl Autonoma Mexico, Biomed Res Inst, Dept Mol Biol & Biotechnol, UNAM, Mexico City 04510, DF, Mexico
Molecular Biology and Biotechnology Department, Biomedical Research Institute, National University of México (UNAM), México City, 04510, Mexico
Infectomics and Molecular Pathogenesis Department, CINVESTAV-IPN, México City, 07360, Mexico
All Open Access, Gold
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