Non-covalent attachment of silver nanoclusters onto single-walled carbon nanotubes with human serum albumin as linking molecule
Por:
RodriguezGalvan, A, Heredia, A, AmelinesSarria, O, Rivera, M, Medina, LA, Basiuk, VA
Publicada:
15 mar 2015
Categoría:
Surfaces, Coatings and Films
Resumen:
Abstract The attachment of silver nanoclusters (AgNCs) onto single-walled carbon nanotubes (SWNTs) for the formation of integrated fluorescence sites has attracted much attention due their potential applications as biological probes and nanovectors in theragnosis. Here, we report the preparation through assembly of fluorescent quasi 1-D nanomaterial based on SWNTs and silver nanoclusters (AgNCs) non-covalently attached to human serum albumin as biological linker. The fluorescent SWNT-AgNCs-HSA conjugates were characterized by atomic force microscopy, high-resolution transmission electron microscopy (HRTEM), high angle annular dark field scanning TEM (HAADF-STEM), fluorescent and UV-vis spectroscopy. The above techniques confirmed that AgNCs were non-covalently attached onto the external surface of SWNTs. In addition, it was observed that the modification did not affect the optical properties of the synthesized AgNCs since the absorption spectra and fluorescence under UV irradiation (? = 365 nm) remain the same. The effect of the functionalized systems was tested on mammal red blood cells (RBCs) and it was found that their structural integrity was compromised by the conjugates, limiting their biological and medical applications. © 2014 Elsevier B.V. All rights reserved.
Filiaciones:
RodriguezGalvan, A:
Univ Nacl Autonoma Mexico, Inst Nacl Cancerol, Unidad Invest Biomed Canc INCan, Mexico City 14080, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Fis, Dept Fis Expt, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Ciencias Nucl, Circuito Exterior CU, Mexico City 04510, DF, Mexico
Heredia, A:
Univ Nacl Autonoma Mexico, Inst Ciencias Nucl, Circuito Exterior CU, Mexico City 04510, DF, Mexico
AmelinesSarria, O:
Univ Nacl Autonoma Mexico, Inst Fis, Dept Mat Condensada, Mexico City 04510, DF, Mexico
Rivera, M:
Univ Nacl Autonoma Mexico, Inst Fis, Dept Mat Condensada, Mexico City 04510, DF, Mexico
Medina, LA:
Univ Nacl Autonoma Mexico, Inst Nacl Cancerol, Unidad Invest Biomed Canc INCan, Mexico City 14080, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Fis, Dept Fis Expt, Mexico City 04510, DF, Mexico
Basiuk, VA:
Univ Nacl Autonoma Mexico, Inst Ciencias Nucl, Circuito Exterior CU, Mexico City 04510, DF, Mexico
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