Oral immunization against porcine pleuropneumonia using the phase of monoolein and purified toxins of Actinobacillus pleuropneumoniae
Por:
Lopez-Bermudez, J, Quintanar-Guerrero, D, Puente, HL, Perez, JT, Guemez, FS, Carrasco, AC, Elvira, SM
Publicada:
28 nov 2014
Resumen:
The main goal of this work was to obtain an orally administered
immunogen that would protect against infections by Actinobacillus
pleuropneumoniae. The Apx I, II and III toxins were obtained from the
supernatants of cultures of serotypes 1 and 3 of A. pleuropneumoniae.
The capacity of monoolein gel to trap and protect the Apx toxins, and
the effect of their incorporation on the stability of the cubic phase
were evaluated. The gel was capable of trapping a 400-mu g/ml
concentration of the antigen with no effects on its structure.
Approximately 60% of the protein molecules were released from the gel
within 4h. Four experimental groups were formed, each one with four
pigs. All challenges were conducted in a nebulization chamber. Group A:
Control (-) not vaccinated and not challenged; Group B: Control (+) not
vaccinated but challenged; Group C: vaccinated twice intramuscularly
with ToxCom (a commercial toxoid) at an interval of 15 days and then
challenged; and Group D: vaccinated orally twice a week for 4 weeks with
ToxOral (an oral toxoid) and challenged on day 28 of the experiment with
a same dose of 2.0 x 10(4) UFC of A. pleuropneumoniae serotypes 1 and 3.
The lesions found in group B covered 27.7-43.1% of the lungs; the pigs
in group C had lesions over 12.3-28%; and those in group D over
15.4-32.3%. No lesions were found in the Group A pigs. A.
pleuropneumoniae induced macroscopic lesions characteristic of infection
by and lesions microscopic detected by histopathology. The etiologic
agent was recovered from the infected lungs, tonsils and spleen. The
serotypes identified were 1 and 3. An indirect ELISA test identified the
antibodies against the Apx toxins in the serum of the animals immunized
orally. (C) 2014 Published by Elsevier Ltd.
Filiaciones:
Lopez-Bermudez, J:
UNAM, FES Cuautitlan, Virol Lab, Cuautitlan 54700, Estado De Mexic, Mexico
Quintanar-Guerrero, D:
UNAM, FES Cuautitlan, Lab Tecnol Farmaceut, Cuautitlan 54700, Estado De Mexic, Mexico
Perez, JT:
UNAM, FES Cuautitlan, Lab Patol, Cuautitlan 54700, Estado De Mexic, Mexico
Guemez, FS:
UNAM, Fac Med Vet & Zootecnia, Cuautitlan 54700, Estado De Mexic, Mexico
Carrasco, AC:
UNAM, FES Cuautitlan, Virol Lab, Cuautitlan 54700, Estado De Mexic, Mexico
Elvira, SM:
UNAM, FES Cuautitlan, Virol Lab, Cuautitlan 54700, Estado De Mexic, Mexico
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