Oestradiol and progesterone differentially alter cytoskeletal protein expression and flame cell morphology in Taenia crassiceps
Por:
Ambrosio J.R., Ostoa-Saloma P., Palacios-Arreola M.I., Ruíz-Rosado A., Sánchez-Orellana P.L., Reynoso-Ducoing O., Nava-Castro K.E., Martínez-Velázquez N., Escobedo G., Ibarra-Coronado E.G., Valverde-Islas L., Morales-Montor J.
Publicada:
1 sep 2014
Resumen:
We examined the effects of oestradiol (E-2) and progesterone (P-4) on
cytoskeletal protein expression in the helminth Taenia crassiceps
specifically actin, tubulin and myosin. These proteins assemble into
flame cells, which constitute the parasite excretory system. Total
protein extracts were obtained from E-2- and P-4-treated T. crassiceps
cysticerci and untreated controls, and analysed by one- and
two-dimensional protein electrophoresis, flow cytometry,
immunofluorescence and videomicroscopy. Exposure of T. crassiceps
cysticerci to E-2 and P-4 induced differential protein expression
patterns compared with untreated controls. Changes in actin, tubulin and
myosin expression were confirmed by flow cytometry of parasite cells and
immunofluorescence. In addition, parasite morphology was altered in
response to E-2 and P-4 versus controls. Flame cells were primarily
affected at the level of the ciliary tuft, in association with the
changes in actin, tubulin and myosin. We conclude that oestradiol and
progesterone act directly on T. crassiceps cysticerci, altering actin,
tubulin and myosin expression and thus affecting the assembly and
function of flame cells. Our results increase our understanding of
several aspects of the molecular crosstalk between host and parasite,
which might be useful in designing anthelmintic drugs that exclusively
impair parasitic proteins which mediate cell signaling and pathogenic
reproduction and establishment. (C) 2014 Australian Society for
Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Filiaciones:
Ambrosio J.R.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Ostoa-Saloma P.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Palacios-Arreola M.I.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Ruíz-Rosado A.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Sánchez-Orellana P.L.:
Departamento de Fisiología Biofísica y Neurociencias, CINVESTAV-IPN, Av. Instituto Politecnico Nacional 2508, San Pedro Zacatenco, Gustavo A. Madero, México DF 07360, Mexico
Reynoso-Ducoing O.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Nava-Castro K.E.:
Inst Nacl Salud Publ, Ctr Invest Enfermedades Infecciosas, Cuernavaca 62100, Morelos, Mexico
Martínez-Velázquez N.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Escobedo G.:
Unidad de Medicina Experimental, Hospital General de México, AP 06726, México DF, Mexico
Ibarra-Coronado E.G.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Valverde-Islas L.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Morales-Montor J.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
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