Cloning, characterization and functional expression of Taenia solium 17 beta-hydroxysteroid dehydrogenase
Por:
Aceves-Ramos A., de la Torre P., Hinojosa L., Ponce A., García-Villegas R., Laclette J.P., Bobes R.J., Romano M.C.
Publicada:
1 jul 2014
Categoría:
Endocrinology
Resumen:
The 17ß-hydroxysteroid dehydrogenases (17ß-HSD) are key enzymes involved in the formation (reduction) and inactivation (oxidation) of sex steroids. Several types have been found in vertebrates including fish, as well as in invertebrates like Caenorhabditis elegans, Ciona intestinalis and Haliotis diversicolor supertexta. To date limited information is available about this enzyme in parasites. We showed previously that Taenia solium cysticerci are able to synthesize sex steroid hormones in vitro when precursors are provided in the culture medium. Here, we identified a T. solium 17ß-HSD through in silico blast searches in the T. solium genome database. This coding sequence was amplified by RT-PCR and cloned into the pcDNA 3.1(+) expression vector. The full length cDNA contains 957. bp, corresponding to an open reading frame coding for 319 aa. The highest identity (84%) at the protein level was found with the Echinococcus multilocularis 17ß-HSD although significant similarities were also found with other invertebrate and vertebrate 17ß-HSD sequences. The T. solium Tsol-17ßHSD belongs to the short-chain dehydrogenase/reductase (SDR) protein superfamily.HEK293T cells transiently transfected with Tsol17ß-HSD induced expression of Tsol17ß-HSD that transformed 3H-androstenedione into testosterone. In contrast, 3H-estrone was not significantly transformed into estradiol. In conclusion, T. solium cysticerci express a 17ß-HSD that catalyzes the androgen reduction. The enzyme belongs to the short chain dehydrogenases/reductase family and shares motifs and activity with the type 3 enzyme of some other species. © 2014 Elsevier Inc.
Filiaciones:
Aceves-Ramos A.:
Dpto. de Fisiología, Biofísica y Neurociencias, CINVESTAV del I.P.N., Apdo. Postal 14-740, 07000 México, D.F., Mexico
de la Torre P.:
UNAM, Dept Immunol, Inst Invest Biomed, Mexico City, DF, Mexico
Hinojosa L.:
Dpto. de Fisiología, Biofísica y Neurociencias, CINVESTAV del I.P.N., Apdo. Postal 14-740, 07000 México, D.F., Mexico
Ponce A.:
Dpto. de Fisiología, Biofísica y Neurociencias, CINVESTAV del I.P.N., Apdo. Postal 14-740, 07000 México, D.F., Mexico
García-Villegas R.:
Dpto. de Fisiología, Biofísica y Neurociencias, CINVESTAV del I.P.N., Apdo. Postal 14-740, 07000 México, D.F., Mexico
Laclette J.P.:
UNAM, Dept Immunol, Inst Invest Biomed, Mexico City, DF, Mexico
Bobes R.J.:
UNAM, Dept Immunol, Inst Invest Biomed, Mexico City, DF, Mexico
Romano M.C.:
Dpto. de Fisiología, Biofísica y Neurociencias, CINVESTAV del I.P.N., Apdo. Postal 14-740, 07000 México, D.F., Mexico
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