Physicochemical and functional characterization of the collagen- polyvinylpyrrolidone copolymer
Por:
Leyva-Gómez G., Lima E., Krotzsch, G, Pacheco-Marín R., Rodríguez-Fuentes N., Quintanar-Guerrero D., Krotzsch, E
Publicada:
7 ago 2014
Resumen:
Collagen-polyvinylpyrrolidone (C-PVP) is a copolymer that is generated from the ? irradiation of a mixture of type I collagen and low-molecular-weight PVP. It is characterized by immunomodulatory, fibrolytic, and antifibrotic properties. Here, we used various physicochemical and biological strategies to characterize the structure, biochemical susceptibility, as well as its effects on metabolic activity in fibroblasts. C-PVP contained 16 times more PVP than collagen, but only 55.8% of PVP was bonded. Nevertheless, the remaining PVP exerted strong structural activity due to the existence of weak bonds that provided shielding in the NMR spectra. On SEM and AFM, freeze-dried C-PVP appeared as a film that uniformly covered the collagen fibers. Size analysis revealed the presence of abundant PVP molecules in the solution of the copolymer with a unique dimension related to macromolecular combinations. Calorimetric analysis showed that the copolymer in solution exhibited structural changes at 110 °C, whereas the lyophilized form showed such changes at temperatures below 50 °C. The copolymer presented a rheopectic behavior, with a predominant effect of the collagen. C-PVP had biological effects on the expression of integrin a2 and prolyl-hydroxylase but did not interact with cells through the collagen receptors because it did not inhibit or slow contraction. © 2014 American Chemical Society.
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Cuernavaca 62210, Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Mat, Dept Mat Met & Ceram, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Lab Invest & Posgrado Tecnol Farmaceut, Cuautitlan 54740, Estado De Mexic, Mexico
Laboratory of Connective Tissue, Centro Nacional de Investigación y Atención de Quemados, Instituto Nacional de Rehabilitación, Calzada Mexico-Xochimilco, Colonia Arenal de Guadalupe, Delegación Tlalpan, 14389 Mexico City, Mexico
Laboratory of Molecular Biology, Instituto Nacional de Medicina Genómica, Periférico Sur 4124, Col. Ex-Rancho de Anzaldo, Torre Zafiro II 5to piso, Mexico City, Mexico
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