Distribution of immune response cells in the pelvic urethra and the prepuce of rams
Por:
Acosta-Dibarrat J., Buendía-Jiménez A., Soriano-Vargas E., de Oca-Jiménez R.M., Tórtora-Pérez J.
Publicada:
1 mar 2014
Categoría:
Veterinary (miscellaneous)
Resumen:
The pathogens of the reproductive system in the male can penetrate and
establish by ascending route, from to the prepuce to the urethra,
accessory glands, epididymis and testicles. The aim of this paper is
determine the distribution and number of cells involved in the immune
response in prepuce and pelvic urethra of rams, without apparent
clinical alterations in testicle, epididymis and prepuce. The
distribution of some of the cells involved in the immune response at the
level of the prepuce and the pelvic urethra was quantified in four
one-year-old rams seronegative for B. ovis and A. seminis and without
apparent lesions in the testicles, the epididymis, and the prepuce. At
the moment of slaughter, samples were taken from the preputial fornix
and the pelvic urethra and placed in 10% formalin and under freezing
conditions. CD4, CD8, WC1, CD45RO, CD14 and CD1b cells were demonstrated
by immunohistochemistry, and immunoglobulin-containing cells (ICC) of
the IgA, IgG and IgM classes were demonstrated by immunofluorescence.
The labeled cells present in the mucosa of both organs were counted with
an image analyzer. The total number of cells was compared between both
tissues and differentially between the epithelium and the connective
tissue of the mucosa. Significant differences were found in the total
number of CD4, CD45RO, and WC1 lymphocytes, in CD14 macrophages, and
CD1b dendritic cells, with mean values being greater in the fornix than
in the urethra (p<0.05) in all cases. Only dendritic cells were found in
the prepuce. No differences were found in the number of CD8 lymphocytes
between both organs. The ratio between each cell type in the connective
and the intraepithelial tissues and between organs was 10/1 for CD4 in
the fornix (p<0.05), against 7/1 in the urethra (p<0.05), while CD8 had
a 1/1 distribution in both mucosae. The WC1 ratio was 5/1 in both
mucosae (p<0.05). CD45RO labeling was 19/1 in the prepuce (p<0.05) and
1/1 in the urethra. IgA-containing cells did not show differences in the
total number of cells in both tissues. In the urethra, no IgG-containing
cells were observed and IgM-containing cells were scarce; in contrast,
both cell types were present in the prepuce, in amounts greater than in
the urethra (p<0.05). IgA-, IgG-, and IgM-containing cells were located
in both organs in the mucosal connective tissue. The presence of
antigen-presenting cells, macrophages, and dendritic cells, as well as
of lymphocytes CD4, CD8 TCR gamma delta (WC1), IgA-, IgG and IgM
positive cells, and CD45RO cells suggests that both mucosae may behave
as inductive and effector sites for the mucosal immune response.
Filiaciones:
Acosta-Dibarrat J.:
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca-Atlacomulco, C.P. 50100 Toluca, Estado de México, México, Mexico
Buendía-Jiménez A.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Cuautitlan 54714, Estado De Mexic, Mexico
Universidad Nacional Autónoma de México, Km 2.5 Carretera Cuautitlán-Teoloyucan, C.P. 54714, Cuautitlán Izcalli, Mexico
Soriano-Vargas E.:
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca-Atlacomulco, C.P. 50100 Toluca, Estado de México, México, Mexico
de Oca-Jiménez R.M.:
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca-Atlacomulco, C.P. 50100 Toluca, Estado de México, México, Mexico
Tórtora-Pérez J.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Cuautitlan 54714, Estado De Mexic, Mexico
Universidad Nacional Autónoma de México, Km 2.5 Carretera Cuautitlán-Teoloyucan, C.P. 54714, Cuautitlán Izcalli, Mexico
Gold
|