Human Mesenchymal Stromal Cells from Adult and Neonatal Sources: A Comparative In Vitro Analysis of Their Immunosuppressive Properties Against T Cells
Por:
Castro-Manrreza, ME, Mayani, H, Monroy-Garcia, A, Flores-Figueroa, E, Chavez-Rueda, K, Legorreta-Haquet, V, Santiago-Osorio, E, Montesinos, JJ
Publicada:
1 jun 2014
Resumen:
Bone marrow-mesenchymal stromal cells (BM-MSCs) have immunosuppressive
properties and have been used in cell therapies as immune regulators for
the treatment of graft-versus-host disease. We have previously
characterized several biological properties of MSCs from placenta (PL)
and umbilical cord blood (UCB), and compared them to those of BM-the
gold standard. In the present study, we have compared MSCs from BM, UCB,
and PL in terms of their immunosuppressive properties against lymphoid
cell populations enriched for CD3(+) T cells. Our results confirm the
immunosuppressive potential of BM-MSCs, and demonstrate that MSCs from
UCB and, to a lesser extent PL, also have immunosuppressive potential.
In contrast to PL-MSCs, BM-MSCs and UCB-MSCs significantly inhibited the
proliferation of both CD4(+) and CD8(+) activated T cells in a cell-cell
contact-dependent manner. Such a reduced proliferation in cell
cocultures correlated with upregulation of programmed death ligand 1 on
MSCs and cytotoxic T lymphocyte-associated Ag-4 (CTLA-4) on T cells, and
increased production of interferon-g, interleukin-10, and prostaglandin
E2. Importantly, and in contrast to PL-MSCs, both BM-MSCs and UCB-MSCs
favored the generation of T-cell subsets displaying a regulatory
phenotype CD4(+) CD25(+) CTLA-4(+). Our results indicate that, besides
BM-MSCs, UCB-MSCs might be a potent and reliable candidate for future
therapeutic applications.
Filiaciones:
Castro-Manrreza, ME:
Univ Nacl Autonoma Mexico, Program Biol Sci, Mexico City 04510, DF, Mexico
Santiago-Osorio, E:
Univ Nacl Autonoma Mexico, FES Zaragoza, Hematopoiesis & Leukemia Lab, Mexico City, DF, Mexico
All Open Access; Green
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