Crystal structure of the shrimp proliferating cell nuclear antigen: Structural complementarity with WSSV DNA polymerase PIP-Box
Por:
Carrasco-Miranda J.S., Lopez-Zavala A.A., Arvizu-Flores A.A., Garcia-Orozco K.D., Stojanoff V., Rudino-Pinera, E, Brieba L.G., Sotelo-Mundo R.R.
Publicada:
11 abr 2014
Resumen:
DNA replication requires processivity factors that allow replicative DNA polymerases to extend long stretches of DNA. Some DNA viruses encode their own replicative DNA polymerase, such as the white spot syndrome virus (WSSV) that infects decapod crustaceans but still require host replication accessory factors. We have determined by X-ray diffraction the threedimensional structure of the Pacific white leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member of the sliding clamp family of proteins, that binds DNA replication and DNA repair proteins through a motif called PIP-box (PCNA-Interacting Protein). The crystal structure of LvPCNA was refined to a resolution of 3 AA , and allowed us to determine the trimeric protein assembly and details of the interactions between PCNA and the DNA. To address the possible interaction between LvPCNA and the viral DNA polymerase, we docked a theoretical model of a PIPbox peptide from the WSSV DNA polymerase within LvPCNA crystal structure. The theoretical model depicts a feasible model of interaction between both proteins. The crystal structure of shrimp PCNA allows us to further understand the mechanisms of DNA replication processivity factors in non-model systems. © 2014 Carrasco-Miranda et al.
Filiaciones:
Carrasco-Miranda J.S.:
Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo Sonora, Mexico
Lopez-Zavala A.A.:
Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo Sonora, Mexico
Arvizu-Flores A.A.:
Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, Mexico
Garcia-Orozco K.D.:
Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo Sonora, Mexico
Stojanoff V.:
National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY, United States
Rudino-Pinera, E:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Med Mol & Bioproc, Cuernavaca 62191, Morelos, Mexico
Brieba L.G.:
Laboratorio Nacional de Genómica Para la Biodiversidad, Centro de Investigación y Estudios Avanzados, Irapuato, Guanajuato, Mexico
Sotelo-Mundo R.R.:
Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo Sonora, Mexico
All Open Access, Gold
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