Analysis of proteomic changes in colored mutants of Xanthophyllomyces dendrorhous (Phaffia rhodozyma)
Por:
Barbachano-Torres A., Castelblanco-Matiz L.M., Ramos-Valdivia A.C., Cerda-García-Rojas C.M., Salgado L.M., Flores-Ortiz C.M., Ponce-Noyola T.
Publicada:
1 jun 2014
Resumen:
The yeast Xanthophyllomyces dendrorhous synthesizes astaxanthin as its
most prevalent xanthophyll derivative. Comparisons between the protein
profiles of mutant lines of this yeast can provide insight into the
carotenogenic pathway. Differently colored mutants (red, orange, pink,
yellow, and white) were obtained from this yeast species, and their
protein profiles were determined using two-dimensional polyacrylamide
gel electrophoresis (2DE). Individual proteins differentially expressed
were identified using mass spectrometry. The red mutants hyperproduced
total carotenoids (mainly astaxanthin), while in white and orange
mutants, mutagenesis affected the phytoene dehydrogenase activity as
indicated by the accumulation of phytoene. Inactivation of astaxanthin
synthase after the mutagenic treatment was evident in beta-carotene
accumulating mutants. Differences in the proteomic profiles of wild-type
X. dendrorhous and its colored mutants were demonstrated using 2DE. Of
the total number of spots detected in each gel (297-417), 128 proteins
were present in all strains. The red mutant showed the greatest number
of matches with respect to the wild type (305 spots), while the white
and yellow mutants, which had reduced concentrations of total
carotenoids, presented the highest correlation coefficient (0.6) between
each other. A number of differentially expressed proteins were
sequenced, indicating that tricarboxylic acid cycle and stress response
proteins are closely related to the carotenogenic process.
Filiaciones:
Barbachano-Torres A.:
Department of Biotechnology and Bioengineering, CINVESTAV-IPN, Av. Instituto Politécnico Nacional # 2508, Zacatenco, Gustavo A. Madero, 07360 Mexico City, Mexico
Castelblanco-Matiz L.M.:
Department of Biotechnology and Bioengineering, CINVESTAV-IPN, Av. Instituto Politécnico Nacional # 2508, Zacatenco, Gustavo A. Madero, 07360 Mexico City, Mexico
Ramos-Valdivia A.C.:
Department of Biotechnology and Bioengineering, CINVESTAV-IPN, Av. Instituto Politécnico Nacional # 2508, Zacatenco, Gustavo A. Madero, 07360 Mexico City, Mexico
Cerda-García-Rojas C.M.:
Department of Chemistry, CINVESTAV-IPN, 07360 Mexico City, Mexico
Salgado L.M.:
CICATA-Qro, Instituto Politécnico Nacional, 76090 Mexico City, Mexico
Flores-Ortiz C.M.:
FES Iztacala UNAM, Mexico City 54090, DF, Mexico
Ponce-Noyola T.:
Department of Biotechnology and Bioengineering, CINVESTAV-IPN, Av. Instituto Politécnico Nacional # 2508, Zacatenco, Gustavo A. Madero, 07360 Mexico City, Mexico
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