Entamoeba histolytica and E. dispar calreticulin: Inhibition of classical complement pathway and differences in the level of expression in amoebic liver abscess
Por:
Ximénez C., González E., Nieves M.E., Silva-Olivares A., Shibayama M., Galindo-Gómez S., Escobar-Herrera J., de Leon, MDG, Morán P., Valadez A., Rojas L., Hernández E.G., Partida O., Cerritos R.
Publicada:
1 ene 2014
Resumen:
The role of calreticulin (CRT) in host-parasite interactions has recently become an important area of research. Information about the functions of calreticulin and its relevance to the physiology of Entamoeba parasites is limited. The present work demonstrates that CRT of both pathogenic E. histolytica and nonpathogenic E. dispar species specifically interacted with human C1q inhibiting the activation of the classical complement pathway. Using recombinant EhCRT protein, we demonstrate that CRT interaction site and human C1q is located at the N-terminal region of EhCRT. The immunofluorescence and confocal microscopy experiments show that CRT and human C1q colocalize in the cytoplasmic vesicles and near to the surface membrane of previously permeabilized trophozoites or are incubated with normal human serum which is known to destroy trophozoites. In the presence of peripheral mononuclear blood cells, the distribution of EhCRT and C1q is clearly over the surface membrane of trophozoites. Nevertheless, the level of expression of CRT in situ in lesions of amoebic liver abscess (ALA) in the hamster model is different in both Entamoeba species; this molecule is expressed in higher levels in E. histolytica than in E. dispar. This result suggests that EhCRT may modulate some functions during the early moments of the host-parasite relationship. © 2014 Cecilia Ximénez et al.
Filiaciones:
Ximénez C.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
González E.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Nieves M.E.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Silva-Olivares A.:
Departamento de Infectómica y Patogénesis Molecular, CINVESTAV, 07360 México, DF, Mexico
Shibayama M.:
Departamento de Infectómica y Patogénesis Molecular, CINVESTAV, 07360 México, DF, Mexico
Galindo-Gómez S.:
Departamento de Infectómica y Patogénesis Molecular, CINVESTAV, 07360 México, DF, Mexico
Escobar-Herrera J.:
Departamento de Biología Celular, CINVESTAV, 07360 México, DF, Mexico
de Leon, MDG:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Morán P.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Valadez A.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Rojas L.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Hernández E.G.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Partida O.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
Cerritos R.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
All Open Access, Gold
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