Whole-brain irradiation increases NREM sleep and hypothalamic expression of IL-1ß in rats
Por:
Ballesteros-Zebadua P., Custodio V., Franco-Perez J., Rubio C., González E., Trejo C., Celis M.A., Paz, C
Publicada:
1 feb 2014
Resumen:
Purpose: Although it has mainly been described qualitatively, whole brain irradiation induces somnolence in patients with malignant diseases. Therefore, we used a rat model to quantify the effects of irradiation of healthy brain tissue on both sleep-wake patterns and the expression of the pro-inflammatory cytokine interleukin-1ß (IL-1ß), which is known to induce sleep. Materials and methods: Different groups were examined at three time points after irradiation (1 day, 30 days and 60 days). Polysomnographic recordings were performed on each rat before and after total cranial irradiation (12 Gy). IL-1ß protein levels in several brain regions were assessed by enzyme-linked immunosorbent assays, and site-specific immunoreactivity was observed by immunofluorescence. Results: We found that both non-rapid eye movement sleep and IL-1ß protein expression in the hypothalamus increased 30 days after irradiation. Conclusions: Whole brain irradiation increases sleep in our rat model, and this finding is similar to qualitative reports from patients. Because IL-1ß has been proposed as a sleep-promoting molecule, we propose that the polysomnographic results may be attributable, at least in part, to the delayed overexpression of IL-1ß in the hypothalamus. © 2014 Informa UK, Ltd.
Filiaciones:
Ballesteros-Zebadua P.:
Univ Nacl Autonoma Mexico, Mexico City 04510, DF, Mexico
Custodio V.:
Departamento de Neurofisiología, Instituto Nacional de Neurología y Neurocirugía, Col. La Fama, C.P. 14269, Tlalpan, Mexico
Franco-Perez J.:
Laboratorio de Fisiología de la Formación Reticular, Instituto Nacional de Neurología y Neurocirugía, Mexico, Mexico
Rubio C.:
Departamento de Neurofisiología, Instituto Nacional de Neurología y Neurocirugía, Col. La Fama, C.P. 14269, Tlalpan, Mexico
González E.:
Departamento de Neurofisiología, Instituto Nacional de Neurología y Neurocirugía, Col. La Fama, C.P. 14269, Tlalpan, Mexico
Trejo C.:
Laboratorio de Neuroinmunología, Instituto Nacional de Neurología y Neurocirugía, Mexico, Mexico
Celis M.A.:
Unidad de Radioneurocirugía, Instituto Nacional de Neurología y Neurocirugía, Mexico, Mexico
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