Pre-conditioning with CDP-choline attenuates oxidative stress-induced cardiac myocyte death in a hypoxia/reperfusion model
Por:
González-Pacheco H., Méndez-Domínguez A., Hernández S., López-Marure R., Vazquez-Mellado M.J., Aguilar C., Rocha-Zavaleta L.
Publicada:
1 ene 2014
Resumen:
Background. CDP-choline is a key intermediate in the biosynthesis of phosphatidylcholine, which is an essential component of cellular membranes, and a cell signalling mediator. CDP-choline has been used for the treatment of cerebral ischaemia, showing beneficial effects. However, its potential benefit for the treatment of myocardial ischaemia has not been explored yet. Aim. In the present work, we aimed to evaluate the potential use of CDP-choline as a cardioprotector in an in vitro model of ischaemia/reperfusion injury. Methods. Neonatal rat cardiac myocytes were isolated and subjected to hypoxia/reperfusion using the coverslip hypoxia model. To evaluate the effect of CDP-choline on oxidative stress-induced reperfusion injury, the cells were incubated with H2O2 during reperfusion. The effect of CDP-choline pre- and postconditioning was evaluated using the cell viability MTT assay, and the proportion of apoptotic and necrotic cells was analyzed using the Annexin V determination by flow cytometry. Results. Pre- and postconditioning with 50 mg/mL of CDP-choline induced a significant reduction of cells undergoing apoptosis after hypoxia/reperfusion. Preconditioning with CDP-choline attenuated postreperfusion cell death induced by oxidative stress. Conclusion. CDP-choline administration reduces cell apoptosis induced by oxidative stress after hypoxia/reperfusion of cardiac myocytes. Thus, it has a potential as cardioprotector in ischaemia/reperfusion-injured cardiomyocytes. © 2014 Héctor González-Pacheco et al.
Filiaciones:
González-Pacheco H.:
Departamento de Urgencias y Unidad Coronaria, Instituto Nacional de Cardiología Ignacio Chavez, Juan Badiano No. 1, Colonia Seccion 16, 14080 Tlalpan, DF, Mexico
Méndez-Domínguez A.:
Departamento de Neurologia, Instituto Nacional de Cardiología Ignacio Chavez, Juan Badiano No. 1, Colonia Seccion 16, 14080 Tlalpan, DF, Mexico
Hernández S.:
Facultad de Medicina, Universidad Panamericana, Augusto Rodin 498, 03920 Insurgentes Mixcoac, DF, Mexico
López-Marure R.:
Departamento de Biologia Celular, Instituto Nacional de Cardiología Ignacio Chavez, Juan Badiano No. 1, Colonia Seccion 16, 14080 Tlalpan, DF, Mexico
Vazquez-Mellado M.J.:
UNAM, Inst Invest Biomed, Dept Mol Biol & Biotechnol, Coyoacan 04510, DE, Mexico
Aguilar C.:
UNAM, Inst Invest Biomed, Dept Mol Biol & Biotechnol, Coyoacan 04510, DE, Mexico
Rocha-Zavaleta L.:
UNAM, Inst Invest Biomed, Dept Mol Biol & Biotechnol, Coyoacan 04510, DE, Mexico
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