DENV-2 subunit proteins fused to CR2 receptor-binding domain (P28)-induces specific and neutralizing antibodies to the Dengue virus in mice
Por:
García-Machorro J., López-González M., Barrios-Rojas O., Fernández-Pomares C., Sandoval-Montes C., Santos-Argumedo L., Villegas-Sepúlveda N., Gutiérrez-Castañeda B., Cedillo-Barrón L.
Publicada:
1 nov 2013
Resumen:
Domain III (DIII) of the dengue virus (DENV) envelope (E) protein induces strong neutralizing type-specific antibodies. In addition, a region near the fusion loop in domain II (DII) induces the production of cross-reactive antibodies with neutralizing potential. Thus, this study aimed to generate DENV-2 recombinant fusion proteins (i.e., rEII*EIII and rEII*EIII/NS1*) either alone or fused to 3 copies of P28, the minimum CR2-binding domain of the complement protein C3d. The 4 recombinant proteins were generated in a Drosophila melanogaster Schneider 2 (S2) cell system. The expression and secretion of the recombinant proteins were confirmed in vitro using immunofluorescence (IF) and western blot (WB) analyses. Human dengue immune serum samples recognized recombinant proteins. The immunogenicity of the 4 proteins in BALB/c mice was analyzed using ELISA, and the results revealed that the induced specific antibody response was higher in the groups of mice immunized with the P28 fusion prote
Filiaciones:
García-Machorro J.:
Department of Molecular Biomedicine Centre for Research and Advanced Studies (CINVesTaV-IPN), Av. IPN # 2508 col., San-Pedro Zacatenco, D.F. Mexico, Mexico
López-González M.:
Department of Molecular Biomedicine Centre for Research and Advanced Studies (CINVesTaV-IPN), Av. IPN # 2508 col., San-Pedro Zacatenco, D.F. Mexico, Mexico
Barrios-Rojas O.:
Department of Molecular Biomedicine Centre for Research and Advanced Studies (CINVesTaV-IPN), Av. IPN # 2508 col., San-Pedro Zacatenco, D.F. Mexico, Mexico
Fernández-Pomares C.:
Department of Molecular Biomedicine Centre for Research and Advanced Studies (CINVesTaV-IPN), Av. IPN # 2508 col., San-Pedro Zacatenco, D.F. Mexico, Mexico
Sandoval-Montes C.:
Immunology Department National School of Biological Sciences, National Polytechnic Institute Prolongación de Carpio y Plan de Ayala Col., Plutarcoelías Calles, D.F. Mexico, Mexico
Santos-Argumedo L.:
Department of Molecular Biomedicine Centre for Research and Advanced Studies (CINVesTaV-IPN), Av. IPN # 2508 col., San-Pedro Zacatenco, D.F. Mexico, Mexico
Villegas-Sepúlveda N.:
Department of Molecular Biomedicine Centre for Research and Advanced Studies (CINVesTaV-IPN), Av. IPN # 2508 col., San-Pedro Zacatenco, D.F. Mexico, Mexico
Gutiérrez-Castañeda B.:
Mexican Autonomous Natl Univ, UMF FES Iztacala, Immunol Dept, Tlalnepantla Edo, Mexico
Cedillo-Barrón L.:
Department of Molecular Biomedicine Centre for Research and Advanced Studies (CINVesTaV-IPN), Av. IPN # 2508 col., San-Pedro Zacatenco, D.F. Mexico, Mexico
All Open Access, Bronze
|