Immunization with a neural-derived peptide protects the spinal cord from apoptosis after traumatic injury
Por:
Rodríguez-Barrera R., Fernández-Presas A.M., García E., Flores-Romero A., Martinon, S, González-Puertos V.Y., Mestre H., Flores-Dominguez C., Rodriguez-Mata V., Konigsberg, M, Solano S., Ibarra A.
Publicada:
1 ene 2013
Resumen:
Apoptosis is one of the most destructive mechanisms that develop after spinal cord (SC) injury. Immunization with neural-derived peptides (INDPs) such as A91 has shown to reduce the deleterious proinflammatory response and the amount of harmful compounds produced after SC injury. With the notion that the aforementioned elements are apoptotic inducers, we hypothesized that INDPs would reduce apoptosis after SC injury. In order to test this assumption, adult rats were subjected to SC contusion and immunized either with A91 or phosphate buffered saline (PBS; control group). Seven days after injury, animals were euthanized to evaluate the number of apoptotic cells at the injury site. Apoptosis was evaluated using DAPI and TUNEL techniques; caspase-3 activity was also evaluated. To further elucidate the mechanisms through which A91 exerts this antiapoptotic effects we quantified tumor necrosis factor-alpha (TNF-a). To also demonstrate that the decrease in apoptotic cells correlated with a functional improvement, locomotor recovery was evaluated. Immunization with A91 significantly reduced the number of apoptotic cells and decreased caspase-3 activity and TNF-a concentration. Immunization with A91 also improved the functional recovery of injured rats. The present study shows the beneficial effect of INDPs on preventing apoptosis and provides more evidence on the neuroprotective mechanisms exerted by this strategy. © 2013 Roxana Rodríguez-Barrera et al.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Facultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Edo. de México CP 52786, Mexico
Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, DF, CP 09340, Mexico
Centro de Investigación Del Proyecto CAMINA A.C., DF, CP 14050, Mexico
Posgrado en Biología Experimental, Universidad Autónoma Metropolitana Iztapalapa, DF, CP 09340, Mexico
All Open Access, Gold
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