Morphological, morphometrical and molecular (CO1 and ITS) analysis of the rotifer Asplanchna brightwellii from selected freshwater bodies in central Mexico
Por:
Jimenez-Contreras, J, Sarma, SSS, Piedra-Lbarra, E, Calderon-Torres, M, Nandini, S
Publicada:
1 nov 2013
Resumen:
We evaluated different strains of the rotifer Asplanchna brightwellii collected from central Mexico using morphology, morphometry and molecular tools (COl and ITS). Three distinct clonal populations from each of the 3 regions (Mexico City, State of Mexico and State of Guerrero) were established under laboratory conditions. For a given waterbody, morphometric comparisons within the populations of A. brightwellii showed almost stable measurements of trophi and with no statistically significant differences among them (p>0.05). However, asplanchnid body length and width as well as the cyst diametervaried significantly depending on the waterbody from which A. brightwellii was collected. The smallest adults (about 700 urn) were from Valerio Trujano lake (Guerrero State) samples while the largest were from Xochimilco lake. Similar tendencies were reflected in the diameter of resting eggs. In addition, morphologically the cysts of A. brightwellii from the three waterbodies showed slightly different pattern. The number of globular structures on the surface of cysts was smaller for Valerio Trujano strain, while these were larger and less numerous for both Xochimilco and Zumpango strains. The ITS region tree displayed two groups Xochimilco and Valerio Trujano-Zumpango, this analysis did not reflect the morphological grouping; on the contrary the COl gene tree separated the populations according to morphological clusters and location (Xochimilco, Valerio Trujano and Zumpango lakes). When the tree was built using the combination of both ITS and CO1 sequences, the phylogenetic relationships observed on CO1gene were consistent; but showed differences with the relationships observed on ITS region tree (only two groups). © Triveni Enterprises, Lucknow (India).
Filiaciones:
Jimenez-Contreras, J:
Univ Nacl Autonoma Mexico, Mexico City 04510, DF, Mexico
Sarma, SSS:
Univ Nacl Autonoma Mexico, Edificio Unidad Morfofisiol, Lab Zool Acuat, Tlalnepantla 54090, Mexico
Piedra-Lbarra, E:
Univ Nacl Autonoma Mexico, Unidad Biomed, Tlalnepantla 54090, Mexico
Calderon-Torres, M:
Univ Nacl Autonoma Mexico, Unidad Prototipos, Tlalnepantla 54090, Mexico
Nandini, S:
Univ Nacl Autonoma Mexico, Edificio Unidad Morfofisiol, Lab Zool Acuat, Tlalnepantla 54090, Mexico
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