Antioxidant properties of xanthones from Calophyllum brasiliense: Prevention of oxidative damage induced by FeSO4
Por:
Blanco-Ayala T., Lugo-Huitrón R., Serrano-López E.M., Reyes-Chilpa R., Rangel-López E., Pineda B., Medina-Campos O.N., Sánchez-Chapul L., Pinzón E., Cristina, TS, Silva-Adaya D., Pedraza-Chaverrí J., Ríos C., De la Cruz V.P., Torres-Ramos M.
Publicada:
11 oct 2013
Categoría:
Complementary and Alternative Medicine
Resumen:
Background: Reactive oxygen species (ROS) are important mediators in a number of degenerative diseases. Oxidative stress refers to the imbalance between the production of ROS and the ability to scavenge these species through endogenous antioxidant systems. Since antioxidants can inhibit oxidative processes, it becomes relevant to describe natural compounds with antioxidant properties which may be designed as therapies to decrease oxidative damage and stimulate endogenous cytoprotective systems. The present study tested the protective effect of two xanthones isolated from the heartwood of Calophyllum brasilienses against FeSO4-induced toxicity.Methods: Through combinatory chemistry assays, we evaluated the superoxide (O2 {filled circle}-), hydroxyl radical (OH{filled circle}), hydrogen peroxide (H2O2) and peroxynitrite (ONOO-) scavenging capacity of jacareubin (xanthone III) and 2-(3,3-dimethylallyl)-1,3,5,6-tetrahydroxyxanthone (xanthone V). The effect of these xanthones on murine DNA and bovine serum albumin degradation induced by an OH• generator system was also evaluated. Additionally, we investigated the effect of these xanthones on ROS production, lipid peroxidation and glutathione reductase (GR) activity in FeSO4-exposed brain, liver and lung rat homogenates.Results: Xanthone V exhibited a better scavenging capacity for O2 {filled circle}-, ONOO- and OH{filled circle} than xanthone III, although both xanthones were unable to trap H2O2. Additionally, xanthones III and V prevented the albumin and DNA degradation induced by the OH{filled circle} generator system. Lipid peroxidation and ROS production evoked by FeSO4 were decreased by both xanthones in all tissues tested. Xanthones III and V also prevented the GR activity depletion induced by pro-oxidant activity only in the brain.Conclusions: Altogether, the collected evidence suggests that xanthones can play a role as potential agents to attenuate the oxidative damage produced by different pro-oxidants. © 2013 Blanco-Ayala et al.; licensee BioMed Central Ltd.
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Biol, Fac Quim, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Unidad Bioterio, Mexico City 04510, DF, Mexico
UNAM INNN, Fac Med, Unidad Perifer Neurociencias, Mexico City 14269, DF, Mexico
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, S.S.A., Insurgentes Sur 3877, México, DF 14269, Mexico
Instituto de Química, Universidad Nacional Autónoma de México, México, DF 04510, Mexico
Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, S.S.A., México, DF 14269, Mexico
Laboratorio de Neuroinmunología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, S.S.A., México, DF 14269, Mexico
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, México, DF 04510, Mexico
Laboratorio de Bioquímica Muscular, Instituto Nacional de Rehabilitación, S.S.A., México, DF 14389, Mexico
Unidad del Bioterio, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF 04510, Mexico
Unidad Periférica de Neurociencias Facultad de Medicina UNAM-INNN, México, DF 14269, Mexico
All Open Access, Gold
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