Lymphoid Progenitor Cells from Childhood Acute Lymphoblastic Leukemia Are Functionally Deficient and Express High Levels of the Transcriptional Repressor Gfi-1
Por:
Purizaca J., Contreras-Quiroz A., Dorantes-Acosta E., Vadillo E., Arriaga-Pizano L., Fuentes-Figueroa S., Villagomez-Barragán H., Flores-Guzmán P., Alvarado-Moreno A., Mayani H., Meza I., Hernandez R., Huerta-Yepez S., Pelayo R.
Publicada:
1 ene 2013
Resumen:
Acute lymphoblastic leukemia (ALL) is the most frequent malignancy of childhood. Substantial progress on understanding the cell hierarchy within ALL bone marrow (BM) has been recorded in the last few years, suggesting that both primitive cell fractions and committed lymphoid blasts with immature stem cell-like properties contain leukemia-initiating cells. Nevertheless, the biology of the early progenitors that initiate the lymphoid program remains elusive. The aim of the present study was to investigate the ability of lymphoid progenitors from B-cell precursor ALL BM to proliferate and undergo multilineage differentiation. By phenotype analyses, in vitro proliferation assays, and controlled culture systems, the lymphoid differentiation potentials were evaluated in BM primitive populations from B-cell precursor ALL pediatric patients. When compared to their normal counterparts, functional stem and progenitor cell contents were substantially reduced in ALL BM. Moreover, neither B nor NK
Filiaciones:
Purizaca J.:
Mexican Inst Social Secur, Oncol Hosp, Oncol Res Unit, Mexico City 06720, DF, Mexico
Contreras-Quiroz A.:
Mexican Inst Social Secur, Oncol Hosp, Oncol Res Unit, Mexico City 06720, DF, Mexico
Dorantes-Acosta E.:
Federico Gomez Childrens Hosp, Mexico City 06720, DF, Mexico
Vadillo E.:
Mexican Inst Social Secur, Oncol Hosp, Oncol Res Unit, Mexico City 06720, DF, Mexico
Arriaga-Pizano L.:
Immunochemistry Research Unit, Medical Specialties Hospital, Mexican Institute for Social Security, 06720 Mexico City, DF, Mexico
Fuentes-Figueroa S.:
UMAE Victorio de la Fuente Narváez, Mexican Institute for Social Security, 07760 Mexico City, DF, Mexico
Villagomez-Barragán H.:
UMAE Victorio de la Fuente Narváez, Mexican Institute for Social Security, 07760 Mexico City, DF, Mexico
Flores-Guzmán P.:
Mexican Inst Social Secur, Oncol Hosp, Oncol Res Unit, Mexico City 06720, DF, Mexico
Alvarado-Moreno A.:
Carlos McGregor Sanchez Hospital, Mexican Institute for Social Security, 03100 Mexico City, DF, Mexico
Mayani H.:
Mexican Inst Social Secur, Oncol Hosp, Oncol Res Unit, Mexico City 06720, DF, Mexico
Meza I.:
Molecular Biomedicine Program, CINVESTAV, 07360 Mexico City, DF, Mexico
Hernandez R.:
Molecular Biomedicine Program, CINVESTAV, 07360 Mexico City, DF, Mexico
Huerta-Yepez S.:
Federico Gomez Childrens Hosp, Mexico City 06720, DF, Mexico
Pelayo R.:
Mexican Inst Social Secur, Oncol Hosp, Oncol Res Unit, Mexico City 06720, DF, Mexico
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