Effect of the plasmid-DNA vaccination on macroscopic and microscopic damage caused by the experimental chronic trypanosoma cruzi infection in the canine Model
Por:
Rodríguez-Morales O., Carrillo-Sánchez S.C., García-Mendoza H., Aranda-Fraustro A., Ballinas-Verdugo M.A., Alejandre-Aguilar R., Rosales-Encina J.L., Vallejo M., Arce-Fonseca M.
Publicada:
1 ene 2013
Resumen:
The dog is considered the main domestic reservoir for Trypanosoma cruzi infection and a suitable experimental animal model to study the pathological changes during the course of Chagas disease (CD). Vaccine development is one of CD prevention methods to protect people at risk. Two plasmids containing genes encoding a trans-sialidase protein (TcSP) and an amastigote-specific glycoprotein (TcSSP4) were used as DNA vaccines in a canine model. Splenomegaly was not found in either of the recombinant plasmid-immunized groups; however, cardiomegaly was absent in animals immunized only with the plasmid containing the TcSSP4 gene. The inflammation of subendocardial and myocardial tissues was prevented only with the immunization with TcSSP4 gene. In conclusion, the vaccination with these genes has a partial protective effect on the enlargement of splenic and cardiac tissues during the chronic CD and on microscopic hearth damage, since both plasmids prevented splenomegaly but only one avoided cardiomegaly, and the lesions in heart tissue of dog immunized with plasmid containing the TcSSP4 gene covered only subepicardial tissue. © 2013 Olivia Rodríguez-Morales et al.
Filiaciones:
Rodríguez-Morales O.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
Carrillo-Sánchez S.C.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
García-Mendoza H.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
Aranda-Fraustro A.:
Department of Pathological Anatomy, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
Ballinas-Verdugo M.A.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
Alejandre-Aguilar R.:
Department of Parasitology, Escuela Nacional de Ciencias Biológicas Del I.P.N., Prolongación de Carpio y Plan de Ayala, Col. Sto. Tomas, Miguel Hidalgo, 11340 Mexico City, DF, Mexico
Rosales-Encina J.L.:
Department of Infectomics and Molecular Pathogenesis, Centro de Investigación y de Estudios Avanzados Del I.P.N., Avenida Instituto Politécnico Nacional No. 2508, Gustavo A. Madero, 07360 Mexico City, DF, Mexico
Vallejo M.:
Department of Sociomedical Research, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
Arce-Fonseca M.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
All Open Access, Gold
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