A comparative study of fibrinogen adsorption onto metal oxide thin films
Por:
Silva-Bermudez P., Muhl S., Rodil S.E.
Publicada:
1 oct 2013
Categoría:
Surfaces, Coatings and Films
Resumen:
One of the first events occurring upon foreign material-biological medium contact is the adsorption of proteins, which evolution greatly determines the cells response to the material. Protein-surface interactions are a complex phenomenon driven by the physicochemical properties of the surface, protein(s) and liquid medium involve in the interaction. In this article the adsorption of fibrinogen (Fbg) onto Ta2O5, Nb2O5, TiO2 and ZrO2 thin films is reported. The adsorption kinetics and characteristics of the adsorbed fibrinogen layer were studied in situ using dynamic and spectroscopic ellipsometry. The films wettability, surface energy (?LW/AB) and roughness were characterized aiming to elucidate their correlations with Fbg adsorption. The adsorption rate changed accordingly to the film; the fastest adsorption rate and highest Fbg surface mass concentration (G) was observed on ZrO2. The hydrophobic/hydrophilic character of the oxide highly influenced Fbg adsorption. On Ta2O5, Nb2O5 and TiO 2, which were either hydrophilic or in the breaking-point between hydrophilicity and hydrophobicity, G was correlated to the polar component of ?LW/AB and roughness of the surface. On ZrO2, clearly hydrophobic, G increased significantly off the correlation observed for the other films. The results indicated different adsorption dynamics and orientations of the Fbg molecules dependent on the surface hydrophobic/hydrophilic character. © 2013 Elsevier B.V. All rights reserved.
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Invest Mat, CU, Mexico City 04510, DF, Mexico
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