Liver exhibits thermal variations according to the stage of fibrosis progression: A novel use of modulated-differential scanning calorimetry for research in hepatology
Por:
Escobedo G., Arjona-Román J.L., Meléndez-Pérez R., Suárez-Álvarez K., Guzmán C., Aguirre-García J., Gutiérrez-Reyes G., Vivas O., Varela-Fascinetto G., Rodríguez-Romero A., Robles-Díaz G., Kershenobich D.
Publicada:
1 jul 2013
Resumen:
Aim: Liver fibrosis results in a disproportion of the hepatic composition and architecture, characterized by a progressive accumulation of fibrillar proteins at the liver parenchyma. Modulated-differential scanning calorimetry (mDSC) is an experimental methodology able to determine the specific thermal signature from any biological substance, based on the variation in heat flow and heat capacity. As these physicochemical properties are directly influenced by compositional and structural changes, we decided to study the thermal behavior of the liver during fibrosis using mDSC. Methods: Liver fibrosis was induced in rats by bile duct ligation or carbon tetrachloride administration. Degree of liver fibrosis was determined by histological examination using the Masson-trichrome stain, accompanied by hepatic expression of a-smooth muscle actin. The thermal analysis was performed in a modulated-differential scanning calorimeter using 20mg of fresh liver mass. Results: The liver showed a characteristic thermal signature in control animals, which progressively differed among mild (F1), moderate (F2) and advanced (F3-F4) liver fibrosis. For heat flow, the hepatic thermal signature from F3-F4 rats exhibited significant differences when compared with F1, F2 and controls. In terms of heat capacity, liver specimens provided a specific thermal signature for each stage of disease, characterized by a transition temperature onset at 95°C for controls, whereas in F1, F2 and F3-F4 animals this temperature significantly decreased to 93°C, 84°C and 75°C, respectively. Conclusion: Because the liver shows a differential thermal signature according to the degree of fibrosis, mDSC could be a novel tool in the study of liver fibrosis progression. © 2012 The Japan Society of Hepatology.
Filiaciones:
Escobedo G.:
Univ Nacl Autonoma Mexico, Unit Expt Med, Gen Hosp Mexico, Mexico City 06726, DF, Mexico
Arjona-Román J.L.:
Univ Nacl Autonoma Mexico, Sch Higher Studies Cuautitlan, Unit Multidisciplinary Res, Mexico City 06726, DF, Mexico
Meléndez-Pérez R.:
Univ Nacl Autonoma Mexico, Sch Higher Studies Cuautitlan, Unit Multidisciplinary Res, Mexico City 06726, DF, Mexico
Suárez-Álvarez K.:
Univ Nacl Autonoma Mexico, Unit Expt Med, Gen Hosp Mexico, Mexico City 06726, DF, Mexico
Guzmán C.:
Univ Nacl Autonoma Mexico, Unit Expt Med, Gen Hosp Mexico, Mexico City 06726, DF, Mexico
Aguirre-García J.:
Univ Nacl Autonoma Mexico, Gen Hosp Mexico, Sch Med, Unit Pathol, Mexico City 06726, DF, Mexico
Gutiérrez-Reyes G.:
Laboratory for Liver, Pancreas and Motility, Department of Experimental Medicine, School of Medicine, General Hospital of Mexico, México D.F., Mexico
Vivas O.:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 06726, DF, Mexico
Varela-Fascinetto G.:
Univ Nacl Autonoma Mexico, Dept Biomacromol, Inst Chem, Mexico City 06726, DF, Mexico
Rodríguez-Romero A.:
Institute for Chemistry, Department of Biomacromolecules, Universidad Nacional Autónoma de México, México D.F., Mexico
Robles-Díaz G.:
Laboratory for Liver, Pancreas and Motility, Department of Experimental Medicine, School of Medicine, General Hospital of Mexico, México D.F., Mexico
Kershenobich D.:
Laboratory for Liver, Pancreas and Motility, Department of Experimental Medicine, School of Medicine, General Hospital of Mexico, México D.F., Mexico
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