LYMPHATIC ROUTE OF TRANSPORT AND PHARMACOKINETICS OF MICRURUS FULVIUS (CORAL SNAKE) VENOM IN SHEEP
Por:
Paniagua D., Jiménez L., Romero C., Vergara I., Calderón A., Benard M., Bernas M.J., Rilo H., De Roodt A., D'Suze G., Witte M.H., Boyer L., Alagón A.
Publicada:
1 dic 2012
Resumen:
The contribution of the lymphatic system to the absorption and systemic bioavailability of Micrurus fulvius venom after subcutaneous (SC) administration was assessed using a central lymph-cannulated sheep model. Micrurus fulvius venom was administered either by intravenous bolus (IV) or subcutaneous injection (SC) in 12 sheep with and without thoracic duct cannulation and drainage. Venom concentration in serum and lymph was determined by a sandwich enzyme-linked immunosorbent assay (ELISA) in samples collected over a 6-hour period and in tissues harvested at the end of the experiment. Pharmacokinetic parameters were determined by a non-compartmental analysis. In the lymphatic cannulated group, over the 6 hours after the venom was administered, 69% of administered dose was accounted for in blood (45%) and lymph (25%). Negligible levels of venom were detected in organs and urine implying that the steady state observed after SC administration is maintained by a slow absorption process. Co
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
Departamento de Producción Agrícola Y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, México, DF, Mexico
Department of Surgery, University of Arizona, Tucson, AZ, United States
Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela
Venom Immunochemistry, Pharmacology, and Emergency Response (VIPER) Institute, University of Arizona, Tucson, AZ, United States
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