Two Common Bean Genotypes with Contrasting Response to Phosphorus Deficiency Show Variations in the microRNA 399-Mediated PvPHO2 Regulation within the PvPHR1 Signaling Pathway
Por:
Ramírez M., Flores-Pacheco G., Reyes J.L., Álvarez A.L., Drevon J.J., Girard L., Hernández G.
Publicada:
1 abr 2013
Resumen:
Crop production of the important legume, the common bean (Phaseolus
vulgaris), is often limited by low phosphorus (P) in the soil. The
genotypes, BAT477 and DOR364, of the common bean have contrasting
responses to P starvation. Plants from the BAT477 P deficiency tolerant
genotype showed higher phosphate content and root biomass as compared to
the DOR364 plants under P starvation. The PvPHR1 transcription
factor-signaling pathway plays an essential role in the response to P
starvation. PvPHO2, a negative regulator of this pathway, encodes an
ubiquitin E2 conjugase that promotes degradation of P-responsive
proteins and is the target gene of PvmiR399. PvPHO2 is downregulated in
BAT477 plants under P deficiency, while such a response is not observed
in P-starved DOR364 plants. Five putative PvmiR399 binding sites were
identified in the 5' UTR region in both genotypes. While four sites
showed an identical DNA sequence, the fifth (binding site of PvPHO2 one)
showed three base changes and higher complementarity scores in DOR364 as
compared to BAT477. Modified 5'RACE experiments indicated that PvmiR399
binding and/or processing was affected in DOR364 P-starved plants. We
propose that a less efficient cleavage of the PvPHO2 mRNA directed by
PvmiR399 would result in a higher PvPHO2-mediated degradation of
P-responsive proteins in the DOR364 genotype with decreased P deficiency
tolerance.
Filiaciones:
Ramírez M.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62210, Morelos, Mexico
Flores-Pacheco G.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62210, Morelos, Mexico
Reyes J.L.:
UNAM, Inst Biotechnol, Dept Biol Mol Plantas, Cuernavaca 62210, Morelos, Mexico
Álvarez A.L.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62210, Morelos, Mexico
Drevon J.J.:
Institut National de la Recherche Agronomique, UMR Eco and Sols-Ecologie Fonctionnelle, Biogéochimie des Sols and Agroécosystèmes, 2 Place Viala, Montpellier F34060, France
Girard L.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62210, Morelos, Mexico
Hernández G.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62210, Morelos, Mexico
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