Multi-functional analysis of Klebsiella Pneumoniae fimbrial types in adherence and biofilm formation
Por:
Alcántar-Curiel M.D., Blackburn D., Saldana, Z, Gayosso-Vázquez C., Iovine N., De la Cruz M.A., Girón J.A.
Publicada:
15 feb 2013
Resumen:
Klebsiella pneumoniae is an opportunistic pathogen frequently associated with nosocomially acquired infections. Host cell adherence and biofilm formation of K. pneumoniae isolates is mediated by type 1 (T1P) and type 3 (MR/K) pili whose major fimbrial subunits are encoded by the fimA and mrkA genes, respectively. The E. coli common pilus (ECP) is an adhesive structure produced by all E. coli pathogroups and a homolog of the ecpABCDE operon is present in the K. pneumoniae genome. In this study, we aimed to determine the prevalence of these three fimbrial genes among a collection of 69 clinical and environmental K. pneumoniae strains and to establish a correlation with fimbrial production during cell adherence and biofilm formation. The PC R-based survey demonstrated that 96% of the K. pneumoniae strains contained ecpA and 94% of these strains produced ECP during adhesion to cultured epithelial cells. Eighty percent of the strains forming biofilms on glass produced ECP,suggesting that ECP is required, at least in vitro, for expression of these phenotypes. The fim operon was found in 100% of the strains and T1P was detected in 96% of these strains. While all the strains examined contained mrkA, only 57% of them produced MR/K fimbriae, alone or together with ECP.In summary, this study highlights the ability of K. pneumoniae strains to produce ECP,which may represent a new important adhesive structure of this organism. Further, it defines the multi-fimbrial nature of the interaction of this nosocomial pathogen with host epithelial cells and inert surfaces. © 2013 Landes Bioscience.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Med, Dept Expt Med, Mexico City 04510, DF, Mexico
Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F, Mexico
Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States
Division of Infectious Diseases, Department of Medicine, University of Florida College of Medicine and the North Florida/South Georgia Veterans Health System, Gainesville, FL, United States
All Open Access, Bronze
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