At3g08030 transcript: a molecular marker of seed ageing
Por:
Garza-Caligaris, LE, Avendano-Vazquez, AO, Alvarado-Lopez, S, Zuniga-Sanchez, E, Orozco-Segovia, A, Perez-Ruiz, RV, Gamboa-DeBuen, A
Publicada:
1 nov 2012
Categoría:
Plant Science
Resumen:
Prolonged storage generally reduces seed viability and vigour, although the rate of deterioration varies among species and environmental conditions. Here, we suggest a possible ageing molecular marker: At3g08030 mRNA. At3g08030 is a member of the DUF642 highly conserved family of cell-wall-associated proteins that is specific for spermatophytes. At3g08030 expression was performed by RT-PCR and qRT-PCR analysis in seed samples differing in their rate of germination and final germination following a matrix priming and/or controlled deterioration (rapid ageing) treatment. The At3g08030 gene transcript was present during the entire Arabidopsis thaliana plant life cycle and in seeds, during maturation, the ripening period and after germination. Matrix priming treatment increased the rate of germination of control seeds and seeds aged by controlled deterioration. Priming treatments also increased At3g08030 expression. To determine whether the orthologues of this gene are also age markers in
Filiaciones:
Garza-Caligaris, LE:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Avendano-Vazquez, AO:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Alvarado-Lopez, S:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Zuniga-Sanchez, E:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Orozco-Segovia, A:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Perez-Ruiz, RV:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Gamboa-DeBuen, A:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
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