PURIFICATION AND PARTIAL CHARACTERIZATION OF A LECTIN FROM THE PRAWN MACROBRACHIUM AMERICANUM (DECAPODA, PALAEMONIDAE)
Por:
Pereyra A., Alpuche J., Sainz J.C., Zenteno E., Agundis C.
Publicada:
1 sep 2012
Categoría:
Aquatic Science
Resumen:
Lectins play relevant roles in the innate immunity of invertebrates. From the haemolymph of the prawn Macrobrachium americanum Spence Bate, 1868 we purified by affinity chromatography a lectin (MaL) composed of three subunits (86.6, 66.2 and 42.7 kDa), as identified by SDS-PAGE. It is mainly composed of Gly, Ser and Arg, and Ala, Glx and Lys in a minor proportion; it lacks Trp, Cys and carbohydrates. MaL agglutinated mainly rat erythrocytes, rabbit or mouse erythrocytes were agglutinated with lower capacity; whereas erythrocytes from human or other species were not agglutinated. It is specific for N-acetyl-neuraminic acid (sialic acid), N-acetyl-D-glucosamine and N-acetyl-D-galactosamine; sialylated fetuin and bovine submaxillary mucin are also powerful inhibitors of the lectin's activity. After physical daily manipulation of prawns for 7 days, the lectin concentration and the specific activity (haemagglutinating activity/protein concentration) increased more than four-fold over the co
Filiaciones:
Pereyra A.:
Univ Nacl Autonoma Mexico, Sch Med, Immunol Lab, Dept Biochem, Mexico City 04510, DF, Mexico
Alpuche J.:
Univ Nacl Autonoma Mexico, Sch Med, Immunol Lab, Dept Biochem, Mexico City 04510, DF, Mexico
Sainz J.C.:
Aquaculture Department, Interdisciplinary Centre of Investigation for the Integral Regional Development, Unit Sinaloa, IPN, Mexico
Zenteno E.:
Univ Nacl Autonoma Mexico, Sch Med, Immunol Lab, Dept Biochem, Mexico City 04510, DF, Mexico
UNAM UABJO, Multidisciplinary Ctr Invest, Oaxaca, Mexico
Agundis C.:
Univ Nacl Autonoma Mexico, Sch Med, Immunol Lab, Dept Biochem, Mexico City 04510, DF, Mexico
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