Two long non-coding RNAs generated from subtelomeric regions accumulate in a novel perinuclear compartment in Plasmodium falciparum
Por:
Sierra-Miranda M., Delgadillo D.M., Mancio-Silva L., Vargas M., Villegas-Sepulveda N., Martínez-Calvillo S., Scherf A., Hernandez-Rivas R.
Publicada:
1 sep 2012
Resumen:
Chromosome ends have been implicated in the default silencing of clonally variant gene families in the human malaria parasite Plasmodium falciparum. These chromosome regions are organized into heterochromatin, as defined by the presence of a repressive histone H3 lysine 9 trimethylated marker and heterochromatin protein 1. Here, we show that the non-coding subtelomeric region adjacent to virulence genes forms facultative heterochromatin in a cell cycle-dependent manner. We demonstrate that telomere-associated repeat elements (TAREs) and telomeres are transcribed as long non-coding RNAs (IncRNAs) during schizogony. Northern blot assays revealed two classes of IncRNAs: a similar to 4-kb transcript composed of telomere sequences and a TARE-3 element, and a >6-kb transcript composed of 21-bp repeats from TARE-6. These IncRNAs are transcribed by RNA polymerase II as single-stranded molecules. RNA-FISH analysis showed that these IncRNAs form several nuclear foci during the schizont stage, wh
Filiaciones:
Sierra-Miranda M.:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional (IPN), Apartado Postal 14-740, 07360 México, D.F., Mexico
Delgadillo D.M.:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional (IPN), Apartado Postal 14-740, 07360 México, D.F., Mexico
Mancio-Silva L.:
Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France
CNRS URA 2581, 75724 Paris, France
Vargas M.:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional (IPN), Apartado Postal 14-740, 07360 México, D.F., Mexico
Villegas-Sepulveda N.:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional (IPN), Apartado Postal 14-740, 07360 México, D.F., Mexico
Martínez-Calvillo S.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Tlalnepantla 54090, Edo De Mexico, Mexico
Scherf A.:
Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France
CNRS URA 2581, 75724 Paris, France
Hernandez-Rivas R.:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional (IPN), Apartado Postal 14-740, 07360 México, D.F., Mexico
All Open Access; Green
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