Recovery from ischemic acute kidney injury by spironolactone administration
Por:
Sanchez-Pozos, K, Barrera-Chimal, J, Garzon-Muvdi, J, Perez-Villalva, R, Rodriguez-Romo, R, Cruz, C, Gamba, G, Bobadilla, NA
Publicada:
1 ago 2012
Resumen:
Prophylactic mineralocorticoid receptor (MR) antagonism with spironolactone (Sp) in rats completely prevents renal damage induced by ischemia. Because acute renal ischemia cannot typically be predicted, this study was designed to investigate whether Sp could prevent renal injury after an ischemic/reperfusion insult. Six groups of male Wistar rats were studied: rats that received a sham abdominal operation (S); rats that underwent 20 min of ischemia and reperfusion for 24 h (I/R) and four groups of rats treated with Sp (20 mg/kg) 0, 3, 6 or 9 h after ischemia. As expected, I/R resulted in renal dysfunction characterized by a fall in renal blood flow and glomerular filtration rate and severe tubular injury which was confirmed by a significant increase in tubular damage biomarkers including kidney injury molecule-1, heat shock protein 72 and urinary protein excretion. The renal injury induced by I/R was in part due to Rho-kinase, endothelin and angiotensin II type 1 receptor upregulation.
Filiaciones:
Sanchez-Pozos, K:
Univ Nacl Autonoma Mexico, Unidad Fisiol Mol, Dept Med Genom, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Barrera-Chimal, J:
Univ Nacl Autonoma Mexico, Unidad Fisiol Mol, Dept Med Genom, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Garzon-Muvdi, J:
Univ Nacl Autonoma Mexico, Unidad Fisiol Mol, Dept Med Genom, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Perez-Villalva, R:
Univ Nacl Autonoma Mexico, Unidad Fisiol Mol, Dept Med Genom, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Rodriguez-Romo, R:
Univ Nacl Autonoma Mexico, Unidad Fisiol Mol, Dept Med Genom, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Gamba, G:
Univ Nacl Autonoma Mexico, Unidad Fisiol Mol, Dept Med Genom, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Bobadilla, NA:
Univ Nacl Autonoma Mexico, Unidad Fisiol Mol, Dept Med Genom, Inst Invest Biomed, Mexico City 04510, DF, Mexico
All Open Access; Bronze
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