Moonlighting Peptides with Emerging Function
Por:
Plaza, JGR, Rojas, AV, Herrera S., Garza-Ramos G., Larios, AT, Amero C., Granados, GZ, Aguilar, MG, Ortiz, MTL, Gonzalez, CP, Carvajal, SU, Coria R., Diaz, AP, Bredesen D.E., Castro-Obregon S., del Rio G.
Publicada:
13 jul 2012
Resumen:
Hunter-killer peptides combine two activities in a single polypeptide that work in an independent fashion like many other multi-functional, multi-domain proteins. We hypothesize that emergent functions may result from the combination of two or more activities in a single protein domain and that could be a mechanism selected in nature to form moonlighting proteins. We designed moonlighting peptides using the two mechanisms proposed to be involved in the evolution of such molecules (i.e., to mutate non-functional residues and the use of natively unfolded peptides). We observed that our moonlighting peptides exhibited two activities that together rendered a new function that induces cell death in yeast. Thus, we propose that moonlighting in proteins promotes emergent properties providing a further level of complexity in living organisms so far unappreciated.
Filiaciones:
Plaza, JGR:
Univ Nacl Autonoma Mexico, Biochem & Struct Biol Dept, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Rojas, AV:
Univ Nacl Autonoma Mexico, Biochem & Struct Biol Dept, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Herrera S.:
Univ Nacl Autonoma Mexico, Biochem & Struct Biol Dept, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Garza-Ramos G.:
Univ Nacl Autonoma Mexico, Dept Biochem, Fac Med, Mexico City 04510, DF, Mexico
Larios, AT:
Univ Nacl Autonoma Mexico, Biochem & Struct Biol Dept, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Amero C.:
Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Cuernavaca Morelos, Mexico
Granados, GZ:
Univ Nacl Autonoma Mexico, Dept Dev Genet & Mol Physiol, Inst Biotecnol, Cuernavaca 62191, Morelos, Mexico
Aguilar, MG:
Univ Nacl Autonoma Mexico, Dept Mol Genet, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Ortiz, MTL:
Univ Nacl Autonoma Mexico, Biochem & Struct Biol Dept, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Gonzalez, CP:
Univ Nacl Autonoma Mexico, Biochem & Struct Biol Dept, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Carvajal, SU:
Univ Nacl Autonoma Mexico, Dept Mol Genet, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Coria R.:
Univ Nacl Autonoma Mexico, Dept Mol Genet, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Diaz, AP:
Univ Nacl Autonoma Mexico, Dept Mol Genet, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Bredesen D.E.:
Buck Institute for Age Research, Novato, CA, United States
Department of Neurology, University of California San Francisco, San Francisco, CA, United States
Castro-Obregon S.:
Univ Nacl Autonoma Mexico, Dept Dev Genet & Mol Physiol, Inst Biotecnol, Cuernavaca 62191, Morelos, Mexico
del Rio G.:
Univ Nacl Autonoma Mexico, Biochem & Struct Biol Dept, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
All Open Access, Gold
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