AUTOCRINE REGULATION OF PROLACTIN SECRETION BY PROLACTIN VARIANTS RELEASED FROM LACTATING RAT ADENOHYPOPHYSIS
Por:
Mena, F, Navarro, N, Castilla, A
Publicada:
1 abr 2012
Resumen:
Background. Previous work has shown that the in vitro released prolactin (PRL.) from the anterior pituitary (AP) of lactating rats, contains PRL variants i.e., from 7-14 to 70-97 kDa that when incubated With AP lactotrophs of male rats, and of rats in other conditions, they promoted the selective stimulation and/or inhibition of the in vitro release of PRL variants from APs of male rats and of rats in other conditions. Methods. In the present experiments, we sought to determine whether PRL variants, released in vitro from lactotrophs of lactating rats, non-suckled (NS) for 6h or suckled (S) or 15 min after NS, were electroeluted from SDS-PAGE, and then were divided into 6 fractions, would influence the in vitro release of PRL variants from lactotrophs of NS and S rat APs. Results. The results obtained showed that, under non-reducing conditions; the fractions contained PRL variants of 7-23 to 97 kDa, and between 1 and more than 20 ng/mu l of PRL protein. Thus similar amounts of total PR
Filiaciones:
Mena, F:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Dept Neurobiol Celular & Mol, Queretaro 76230, Qro, Mexico
Navarro, N:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Dept Neurobiol Celular & Mol, Queretaro 76230, Qro, Mexico
Castilla, A:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Dept Neurobiol Celular & Mol, Queretaro 76230, Qro, Mexico
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