Taurine in adipocytes prevents insulin-mediated H(2)o(2) generation and activates Pka and lipolysis
Por:
Pina-Zentella, G, de la Rosa-Cuevas, G, Vazquez-Meza, H, Pina, E, de Pina, MZ
Publicada:
1 may 2012
Resumen:
Among many actions assigned to taurine (Tau), the most abundant amino acid in numerous mammalian tissues, it prevents high-fat diet-induced obesity with increasing resting energy expenditure. To sustain this Tau action, the goal of the present study was to explore the acute effects of Tau on baseline and on adrenaline, insulin and their second messengers to modulate lipolysis in white adipose tissue (WAT) cells from rat epididymis. The Tau effects in this topic were compared with those recorded with Gly, Cys and Met. Tau on its own did not modify baseline lipolysis. Tau raised isoproterenol- and dibutyryl-cAMP (Bt(2)cAMP)-activated glycerol release. Gly diminished Bt(2)cAMP-activated glycerol release, and Cys and Met had no effect. Cyclic AMP-dependent activation of protein kinase A (PKA) in cell-free extracts decreased slightly by Gly and was unaltered by Cys, Met, and Tau. PKA catalytic activity in cell-free extracts was stimulated by Tau and unchanged by Cys, Gly and Met. Gly and Ta
Filiaciones:
Univ Nacl Autonoma Mexico, Dept Biochem, Fac Med, Mexico City 04510, DF, Mexico
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