ANCUT2, an Extracellular Cutinase from Aspergillus nidulans Induced by Olive Oil
Por:
Castro-Ochoa, D, Pena-Montes, C, Gonzalez-Canto, A, Alva-Gasca, A, Esquivel-Bautista, R, Navarro-Ocana, A, Farres, A
Publicada:
1 mar 2012
Resumen:
Cutinases are versatile carboxylic ester hydrolases with great potential in many biocatalytic processes, including biodiesel production. Genome sequence analysis of the model organism Aspergillus nidulans reveals four genes encoding putative cutinases. In this work, we purified and identified for the first time a cutinase (ANCUT2) produced by A. nidulans. ANCUT2 is a 29-kDa protein which consists of 255 amino acid residues. Comparison of the amino acid sequence of ANCUT2 with other microbial cutinase sequences revealed a high degree of homology with other fungal cutinases as well as new features, which include a serine-rich region and conserved cysteines. Cutinase production with different lipidic and carbon sources was also explored. Enzyme activity was induced by olive oil and some triacylglycerides and fatty acids, whereas it was repressed by glucose (1%) and other sugars. In some conditions, a 22-kDa post-translational processing product was also detected. The cutinase nature of th
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Chem, Dept Food Sci & Biotechnol, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Hosp Gen Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
|