Sodium caseinate induces mouse granulopoiesis
Por:
Domínguez-Melendez V., Silvestre-Santana O., Moreno-Fierros L., Aguiniga-Sanchez, I, Martínez L., Marroquin-Segura R., García-Hernández A.L., Weiss-Steider B., Marché-Cova A., Monroy-García A., Mora-García L., Santiago-Osorio E.
Publicada:
1 abr 2012
Resumen:
Sodium caseinate (CasNa) induces differentiation and M-CSF production in mouse band granulocytes in vitro; however, it is not yet known if this molecule can also induce the proliferation and activation of the granulocyte lineage in vivo. In this work we evaluated the induction in vivo of granulopoiesis and the activation of granulocytes in mice treated with CasNa. BALB/c male mice 8-12 weeks old were used. The animals were inoculated intraperitoneally with 1 ml of CasNa (10% in PBS p/v) four times (every 48 h). Granulocyte proliferation was evaluated by flow cytometry; activation was evaluated by phagocytic indices. The cytokine was measured using an ELISA assay. We show that CasNa increased bone marrow granulopoiesis percentage (38.35 +/- A 10.88 vs. 64.94 +/- A 34.14 BrdU+/Gr-1+ cells) and the granulocytes generated presented increased phagocytic indices (0.3 +/- A 0.1 vs. 0.6 +/- A 0.11, p < 0.05). We also show that G-CSF (974 +/- A 411 vs. 3189 +/- A 350 pg/ml, p < 0.05) and GM-CSF
Filiaciones:
Domínguez-Melendez V.:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Silvestre-Santana O.:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Moreno-Fierros L.:
UNAM, FES Iztacala, UBIMED, Lab Inmunidad Mucosas, Tlalnepantla, Estado De Mexic, Mexico
Aguiniga-Sanchez, I:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Martínez L.:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Marroquin-Segura R.:
Univ Nacl Autonoma Mexico, Lab Inmunol, Mexico City 09230, DF, Mexico
García-Hernández A.L.:
Univ Nacl Autonoma Mexico, UBIMED, FES Iztacala, Lab Inmunidad Mucosas, Mexico City 09230, DF, Mexico
Weiss-Steider B.:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
Marché-Cova A.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City 04510, DF, Mexico
Monroy-García A.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Inmunobiol, Mexico City 09230, DF, Mexico
Mora-García L.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Inmunobiol, Mexico City 09230, DF, Mexico
Santiago-Osorio E.:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, Mexico City, DF, Mexico
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